Rhodopsin Antibody (HL2668)

Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rhodopsin antibody [HL2668] (NBP3-48717) detects Rhodopsin protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4C for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4C for 1 hour.

Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Unboiled Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, IHC
• Clone: HL2668
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rhodopsin Antibody (HL2668) Summary

• Description: Novus Biologicals Rabbit Rhodopsin Antibody (HL2668) (NBP3-48717) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide encompassing a sequence within the Extracellular domain human of Rhodopsin. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RHO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Rhodopsin Antibody (HL2668)

• CSNBAD1
• MGC138309
• OPN2
• OPN2MGC138311
• opsin 2, rod pigment
• Opsin-2
• retinitis pigmentosa 4, autosomal dominant
• RHO
• Rhodopsin
• RP4

Background

Rhodopsin is a glycoprotein which is a visual pigment in mammals, birds, amphibians, and reptiles. The rhodopsin protein is present on the photoreceptor cells in the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHO