Rhodopsin Antibody (1D4)


Western Blot: Rhodopsin Antibody (Rho 1D4) [NBP1-47602] - Human cell lines using a 1:1000 dilution.
Immunohistochemistry: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary ...read more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
1 mg/ml

Rhodopsin Antibody (1D4) Summary

Bovine Rhodopsin
binds to the C- terminal epitope-T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin
Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins. The antibody specifically binds to the C- terminal epitope
Protein G purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
PBS pH7.4, 50% glycerol
0.09% Sodium Azide
1 mg/ml
Protein G purified

Reactivity Notes

Vertebrate. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.


Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Alternate Names for Rhodopsin Antibody (1D4)

  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4


Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-47602AF405
Alexa Fluor (R) 488 Labeled NBP1-47602AF488
Alexa Fluor (R) 647 Labeled NBP1-47602AF647
Alexa Fluor (R) 700 Labeled NBP1-47602AF700
Allophycocyanin Labeled NBP1-47602APC
DyLight 350 Labeled NBP1-47602UV
DyLight 405 Labeled NBP1-47602V
DyLight 405LS Labeled NBP1-47602V3
DyLight 488 Labeled NBP1-47602G
DyLight 680 Labeled NBP1-47602FR
DyLight 755 Labeled NBP1-47602IR
PE Labeled NBP1-47602PE
PerCP Labeled NBP1-47602PCP

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Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol RHO

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