Rhodopsin Antibody (1D4)

Images

 
Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA ...read more
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, VbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Rhodopsin Antibody (1D4) Summary

Immunogen
Bovine Rhodopsin
Epitope
binds to the C- terminal epitope-T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin
Localization
Membrane
Specificity
Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of Rhodopsin Antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Publications
Read Publications using
NBP1-47602 in the following applications:

  • IHC
    1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28672005).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Alternate Names for Rhodopsin Antibody (1D4)

  • CSNBAD1
  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4

Background

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhodopsin Antibody (NBP1-47602)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IP, WB.


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IHC
(1)
IP
(1)
WB
(2)
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Human
(1)
Mouse
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP1-47602) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-47602AF405
Alexa Fluor 488 NBP1-47602AF488
Alexa Fluor 647 NBP1-47602AF647
Alexa Fluor 700 NBP1-47602AF700
Biotin NBP1-47602B
DyLight 350 NBP1-47602UV
DyLight 405 NBP1-47602V
DyLight 488 NBP1-47602G
DyLight 550 NBP1-47602R
DyLight 650 NBP1-47602C
DyLight 680 NBP1-47602FR
DyLight 755 NBP1-47602IR
FITC NBP1-47602F
HRP NBP1-47602H
Janelia Fluor 549 NBP1-47602JF549
Janelia Fluor 646 NBP1-47602JF646

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Bioinformatics

Gene Symbol RHO
Uniprot