Rhodopsin Antibody (1D4)

Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.

• Reactivity: Hu, Mu, Bv, Vb
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
• Clone: 1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Rhodopsin Antibody (1D4) Summary

• Immunogen: Bovine Rhodopsin
• Epitope: binds to the C- terminal epitope-T-E-T-S-Q-V-A-P-A- (COOH) of rhodopsin
• Localization: Membrane
• Specificity: Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: 1 ug/ml of Rhodopsin Antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28672005).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Notes

Proteins containing the epitope tag can be selectively bound to a Rho 1D4 affinity matrix and eluted using an excess of -T-E-T-S-Q-V-A-P-A- peptide under mild conditions.

Alternate Names for Rhodopsin Antibody (1D4)

• CSNBAD1
• MGC138309
• OPN2MGC138311
• opsin 2, rod pigment
• opsin-2
• retinitis pigmentosa 4, autosomal dominant
• rhodopsin
• RP4

Background

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rhodopsin Antibody (NBP1-47602)(3)

Publications using NBP1-47602

• Daniloski Z, Guo X, Sanjana NE The D614G mutation in SARS-CoV-2 Spike increases transduction of multiple human cell types bioRxiv Jun 15 2020 [PMID: 32587969] (WB, IP, Human)
• Ramachandra Rao S, Fliesler SJ, Kotla P et al. Lack of Overt Retinal Degeneration in a K42E Dhdds Knock-In Mouse Model of RP59 Cells Apr 7 2020 [PMID: 32272552] (IHC, Mouse)
• Cubizolle A, Guillou L, Mollereau B et al. Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina PLoS ONE 2017 Jul 03 [PMID: 28672005] (WB, Mouse)

Bioinformatics

• Gene Symbol: RHO
• Uniprot:
  • P08100(Human)