RhoD Antibody (OTI2F7)

Western Blot: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RhoD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RhoD.

Immunocytochemistry/Immunofluorescence: RhoD Antibody (2F7) [NBP2-01462] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RhoD.

Flow Cytometry: RhoD Antibody (2F7) [NBP2-01462] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RhoD antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI2F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.85 mg/ml

RhoD Antibody (OTI2F7) Summary

• Description: Novus Biologicals Mouse RhoD Antibody (OTI2F7) (NBP2-01462) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RHOD(NP_055393) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 23.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.85 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RhoD Antibody (OTI2F7)

• ARHDRHOM
• ras homolog D
• ras homolog gene family, member A
• ras homolog gene family, member D
• Rho
• RhoD
• RhoHP1
• rho-related GTP-binding protein RhoD
• Rho-related protein HP1

Background

Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOD
• Entrez:
  • 29984(Human)