RhoC Antibody

Western Blot: RhoC Antibody [NBP2-20156] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RhoC Antibody [NBP2-20156] - Sample: A431 cells were fixed in iced-cold MeOH for 5 min. Green: RHOC protein stained by RhoC antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: RhoC Antibody [NBP2-20156] - Immunohistochemical analysis of paraffin-embedded Ovarian carcinoma, using antibody at 1:250 dilution.

Western Blot: RhoC Antibody [NBP2-20156] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE gel, diluted at 1:3000.

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RhoC Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RhoC. The exact sequence is proprietary.
• Localization: Cell membrane; Lipid-anchor; Cytoplasmic side
• Predicted Species: Rat (100%), Chicken (96%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHOC
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RhoC Antibody

• ARH9ARHCRho cDNA clone 9
• H9
• MGC1448
• MGC61427
• oncogene RHO H9
• ras homolog gene family, member C
• RAS-related homolog 9
• rhoC GTPase
• RhoC
• RHOH9
• rho-related GTP-binding protein RhoC
• small GTP binding protein RhoC

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RHOC
• Uniprot:
  • P08134(Human)