RHCG Antibody

Images

 
Western Blot: RHCG Antibody [NBP1-52089] - Analysis of RHCG Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52089 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

RHCG Antibody Summary

Immunogen
Peptide with sequence C-ERQNSVYQSD corresponding to internal region according to NP_057405.1.
Epitope
C-ERQNSVYQSD
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RHCG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1-3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
Application Notes
Western blot: Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Primary incubation was 1 hour
IHC: Paraffin embedded Human Tonsil and Kidney.
Publications
Read Publications using NBP1-52089.

Reactivity Notes

Expected from sequence similarity: Canine, Porcine

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RHCG Antibody

  • ammonium transporter Rh type C
  • CDRC2
  • chromosome 15 open reading frame 6
  • PDRC2C15orf6
  • Rh family type C glycoprotein
  • Rh family, C glycoprotein
  • Rh glycoprotein kidney
  • Rh type C glycoprotein
  • Rhesus blood group family type C glycoprotein
  • Rhesus blood group, C glycoprotein
  • RHGKTumor-related protein DRC2
  • SLC42A3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RHCG Antibody (NBP1-52089)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RHCG
Entrez
Uniprot