RHCG Antibody

Western Blot: RHCG Antibody [NBP1-52089] - Analysis of RHCG Human Testis lysate (35ug protein in RIPA buffer) using NBP1-52089 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RHCG Antibody [NBP1-52089] - 5ug/ml staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RHCG Antibody Summary

• Description: Novus Biologicals Goat RHCG Antibody (NBP1-52089) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-RHCG Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ERQNSVYQSD corresponding to internal region according to NP_057405.1.
• Epitope: C-ERQNSVYQSD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RHCG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100-1:2000
• Application Notes: Western blot: Approx 60kDa band observed in Human Testis lysates (calculated MW of 53.2kDa according to NP_057405.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Biol Chem. 2000 Aug 18;275(33):25641-51;PMID: 10852913). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Tonsil and Kidney.

Reactivity Notes

Expected from sequence similarity: Canine, Porcine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RHCG Antibody

• ammonium transporter Rh type C
• CDRC2
• chromosome 15 open reading frame 6
• PDRC2C15orf6
• Rh family type C glycoprotein
• Rh family, C glycoprotein
• Rh glycoprotein kidney
• Rh type C glycoprotein
• Rhesus blood group family type C glycoprotein
• Rhesus blood group, C glycoprotein
• RHGKTumor-related protein DRC2
• SLC42A3

Background

RHCG functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RHCG Antibody (NBP1-52089)(3)

Publications using NBP1-52089

• Han KH, Mekala K, Babida V et al. Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung. Am J Physiol Cell Physiol 2009-07-01 [PMID: 19429772]
• Liu Z, Chen Y, Mo R et al. Characterization of human RhCG and mouse Rhcg as novel nonerythroid Rh glycoprotein homologues predominantly expressed in kidney and testis. J Biol Chem 2000-08-01 [PMID: 10852913]
• Cotellessa JF Urinary RNA Biomarkers Prognostic for Renal Cell Carcinoma Metastasis Thesis 2021-01-01 (WB)

Bioinformatics

• Gene Symbol: RHCG
• Entrez:
  • 51458(Human)
• Uniprot:
  • NP_057405.1(Human)