RGS14 Antibody - BSA Free

Western Blot: RGS14 Antibody [NBP2-20148] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: RGS14 Antibody [NBP2-20148] - Paraffin-embedded mouse testis. RGS14 antibody [N1N3] dilution: 1:500.

Immunohistochemistry-Paraffin: RGS14 Antibody [NBP2-20148] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: RGS14 Antibody [NBP2-20148] - Paraffin-embedded mouse fore brain. RGS14 antibody [N1N3] dilution: 1:500.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RGS14 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RGS14. The exact sequence is proprietary.
• Localization: Membrane
• Predicted Species: Rat (90%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RGS14
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RGS14 Antibody - BSA Free

• regulator of G-protein signaling 14
• regulator of G-protein signalling 14

Background

This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RGS14
• Uniprot:
  • O43566(Human)