RGS16 Antibody (OTI4E5)

Images

 
Western Blot: RGS16 Antibody (4E5) [NBP2-01584] Analysis of extracts (35ug) from 9 different cell lines by using anti-RGS16 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: RGS16 Antibody (4E5) [NBP2-01584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RGS16.
Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human pancreas tissue using anti-RGS16 mouse monoclonal antibody.
Flow Cytometry: RGS16 Antibody (4E5) [NBP2-01584] - Analysis of Jurkat cells, using anti-RGS16 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: RGS16 Antibody (4E5) [NBP2-01584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGS16 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human breast tissue using anti-RGS16 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human endometrium tissue using anti-RGS16 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: RGS16 Antibody (4E5) [NBP2-01584] - Staining of paraffin-embedded Human Kidney tissue using anti-RGS16 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RGS16 Antibody (OTI4E5) Summary

Immunogen
Full length human recombinant protein of human RGS16(NP_002919) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RGS16
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-01584 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for RGS16 Antibody (OTI4E5)

  • A28-RGS14
  • A28-RGS14P
  • hRGS-r
  • regulator of G-protein signaling 16
  • regulator of G-protein signalling 16
  • Retinally abundant regulator of G-protein signaling
  • Retinal-specific RGS
  • RGSR
  • RGS-r

Background

The protein encoded by this gene belongs to the 'regulator of G protein signaling' family. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits. It also may play a role in regulating the kinetics of signaling in the phototransduction cascade. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RGS16 Antibody (NBP2-01584)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RGS16 Antibody (NBP2-01584) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73881AF350
Alexa Fluor 405 NBP2-73881AF405
Alexa Fluor 488 NBP2-73881AF488
Alexa Fluor 532 NBP2-73881AF532
Alexa Fluor 594 NBP2-73881AF594
Alexa Fluor 647 NBP2-73881AF647
Alexa Fluor 700 NBP2-73881AF700
Alexa Fluor 750 NBP2-73881AF750
Allophycocyanin NBP2-73881APC
Allophycocyanin/Cy7 NBP2-73881APCCY7
Biotin NBP2-73881B
DyLight 350 NBP2-73881UV
DyLight 405 NBP2-73881V
DyLight 488 NBP2-73881G
DyLight 550 NBP2-73881R
DyLight 594 NBP2-73881DL594
DyLight 650 NBP2-73881C
DyLight 680 NBP2-73881FR
DyLight 755 NBP2-73881IR
FITC NBP2-73881F
HRP NBP2-73881H
Janelia Fluor 549 NBP2-73881JF549
Janelia Fluor 646 NBP2-73881JF646
PE NBP2-73881PE
PE/Atto594 NBP2-73881PEATT594
PE/Cy5.5 NBP2-73881PECY55
PE/Cy7 NBP2-73881PECY7
PerCP NBP2-73881PCP

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Bioinformatics

Gene Symbol RGS16
Entrez