RGS14 Antibody

Western Blot: RGS14 Antibody [NB100-1431] - NB100-1431 staining of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (0.5 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RGS14 Antibody Summary

• Description: Novus Biologicals Goat RGS14 Antibody (NB100-1431) is a polyclonal antibody validated for use in WB and ELISA. Anti-RGS14 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-IGGSLNSTTDSAL corresponding to C-Terminus according to NP_006471.2.
• Localization: Cell Membrane (probable)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RGS14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 65-70 kDa band observed in Jurkat lysates (predicted MW of 65 kDa band according to NP_006471).
• Control:

  Jurkat Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RGS14 Antibody

• regulator of G-protein signaling 14
• regulator of G-protein signalling 14

Background

RGS14 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP bound form. Contains 1 GoLoco domain, 2 Ras binding (RBD) domains, 1 RGS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS14 Antibody (NB100-1431)(1)

Publication using NB100-1431

• Snow BE, Antonio L, Suggs S et al. Molecular cloning and expression analysis of rat Rgs12 and Rgs14. Biochem Biophys Res Commun 1997-04-28 [PMID: 9168931]

Bioinformatics

• Gene Symbol: RGS14
• Entrez:
  • 10636(Human)
• Uniprot:
  • NP_006471.(Human)