RFX2 Overexpression Lysate (Native)

Images

 
Western Blot: RFX2 Overexpression Lysate (Adult Normal) [NBL1-15305] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFX2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RFX2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209758 and based on accession number NM_000635. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens regulatory factor X, 2 (influences HLA class II expression) (RFX2), transcript variant 1, mRNA.
Gene
RFX2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
79.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RFX2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RFX2 Overexpression Lysate (Native)

  • DNA binding protein RFX2
  • DNA-binding protein RFX2
  • FLJ14226
  • HLA class II regulatory factor RFX2
  • Regulatory factor X 2
  • regulatory factor X, 2 (influences HLA class II expression)
  • trans-acting regulatory factor 2

Background

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. Transcript Variant: This variant (1) is the longer transcript and encodes the longer protein (isoform a).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-52654
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-86301
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-56042
Species: Hu
Applications: IHC-P
NBP2-48967
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-19461
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
232-FA/CF
Species: Hu
NB100-2475
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA
NBP1-87928
Species: Hu
Applications: IHC, IHC-P
NBP1-90171
Species: Hu
Applications: IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP2-27188
Species: Hu, Po, Ca, Pm
Applications: WB, Simple Western
NBP2-13744
Species: Hu
Applications: IHC, IHC-P
NBP1-88309
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-43538
Species: Hu
Applications: WB, IHC, IHC-P
NBL1-15305
Species: Hu
Applications: WB

Publications for RFX2 Lysate (NBL1-15305) (0)

There are no publications for RFX2 Lysate (NBL1-15305).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RFX2 Lysate (NBL1-15305) (0)

There are no reviews for RFX2 Lysate (NBL1-15305). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RFX2 Lysate (NBL1-15305) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RFX2 Products

Bioinformatics Tool for RFX2 Lysate (NBL1-15305)

Discover related pathways, diseases and genes to RFX2 Lysate (NBL1-15305). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RFX2 Lysate (NBL1-15305)

Discover more about diseases related to RFX2 Lysate (NBL1-15305).
 

Pathways for RFX2 Lysate (NBL1-15305)

View related products by pathway.

PTMs for RFX2 Lysate (NBL1-15305)

Learn more about PTMs related to RFX2 Lysate (NBL1-15305).

Blogs on RFX2

There are no specific blogs for RFX2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

RFX2 Antibody
NBP2-13224

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RFX2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RFX2