Rev-erb beta/NR1D2 Overexpression Lysate

Images

 
Western Blot: NR1D2 Overexpression Lysate (Adult Normal) [NBL1-13766] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NR1D2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Rev-erb beta/NR1D2 Overexpression Lysate Summary

Description
Transient overexpression lysate of nuclear receptor subfamily 1, group D, member 2 (NR1D2), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC206338 and based on accession number NM_005126. The protein contains a C-MYC/DDK Tag.
Gene
NR1D2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
64.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Rev-erb beta/NR1D2 Overexpression Lysate

  • BD73
  • EAR-1R
  • HZF2
  • NR1D2
  • nuclear receptor subfamily 1 group D member 2
  • nuclear receptor subfamily 1, group D, member 2
  • Orphan nuclear hormone receptor BD73
  • Reverb beta
  • Rev-erb beta
  • rev-erba-alpha-related receptor
  • rev-erb-beta
  • RVR
  • V-erbA-related protein 1-related

Background

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Rev-erb beta/NR1D2 Lysate (NBL1-13766) (0)

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Bioinformatics

Gene Symbol NR1D2