RER1 Antibody

Immunohistochemistry-Paraffin: RER1 Antibody [NBP1-00234] - Staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RER1 Antibody Summary

• Description: Novus Biologicals Goat RER1 Antibody (NBP1-00234) is a polyclonal antibody validated for use in IHC and ELISA. Anti-RER1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HGKRRYRGKEDAGK corresponding to C-Terminus according to NP_008964.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave an approx. 45 kDa band in human brain lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.9 kDa band according to NP_008964.3. The 45 kDa band was successfully blocked by incubation with the immunizing peptide. IHC: An anonymous customer has reported successful usage on human frontal cortex.
• Control:

  RER1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RER1 Antibody

• protein RER1
• RER1 retention in endoplasmic reticulum 1 homolog (S. cerevisiae)

Background

The protein encoded by the RER1 gene is a multi-pass membrane protein that is localized to the golgi apparatus. It is involved in the retention of endoplasmic reticulum (ER) membrane proteins in the ER and retrieval of ER membrane proteins from the early Golgi compartment to facilitate gamma-secretase complex assembly. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RER1 Antibody (NBP1-00234)(1)

Publication using NBP1-00234

• C. Kaether, J. Scheuermann, M. Fassler et al. Endoplasmic reticulum retention of the gamma-secretase complex component Pen2 by Rer1. EMBO Rep 2007-08-01 [PMID: 17668005]

Bioinformatics

• Gene Symbol: RER1
• Entrez:
  • 11079(Human)
  • 67830(Mouse)
• Uniprot:
  • NP_008964.3(Human)