REPIN1 Knockout 293T Cell Lysate

Product Discontinued

REPIN1 Knockout 293T Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,13 bp deletion in exon 1 and 2 bp deletion in exon 1
• Gene: REPIN1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout 293T Cell
• Life Stage: Embryonic
• Tissue: Kidney

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for REPIN1 Knockout 293T Cell Lysate

• 60 kDa origin-specific DNA-binding protein
• 60 kDa replication initiation region protein
• ATT-binding protein
• DHFR oribeta-binding protein RIP60
• H_DJ0584D14.12
• replication initiation region protein (60kD)
• replication initiator 1
• RIP60AP4
• Zfp464
• zinc finger protein 464 (RIP60)
• Zinc finger protein 464
• zinc finger protein AP4
• zinc finger proten AP4
• ZNF464

Background

REPIN1, also known as Replication initiator 1, is a 64kDa 567 amino acid protein that can be found in the nucleus. This gene is necessary for the process of initiating the chromosomal DNA replication. REPIN1 has been researched in relation to acrodermatitis enteropathica, enteropathica, acrodermatitis and immunodeficiency. REPIN1 has been linked to the synaptic transmission, cell proliferation, DNA replication, fatty acid transport and cell growth pathways where it interacts with GMNN, HDAC3, HDAC1, HDAC2 and GATA3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: REPIN1