RENT1/UPF1/hUPF1 Antibody

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Whole cell lysate from HeLa, (15 ug), 293T, (15 ug), Jurkat, (15 ug), mouse TCMK-1, (50 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Whole cell lysate from HeLa and mouse NIH3T3.

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody [NBP1-05967] - Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody NBP1-05967 used for IP at 3 ug per reaction. RENT1 was also immunoprecipitated by rabbit anti-RENT1 antibody NB100-370. For blotting immunoprecipitated RENT1, NBP1-05967 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RENT1/UPF1/hUPF1 Antibody Summary

• Description: Novus Biologicals Rabbit RENT1/UPF1/hUPF1 Antibody (NBP1-05967) is a polyclonal antibody validated for use in WB and IP. Anti-RENT1/UPF1/hUPF1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1079 and 1129 of human regulator of nonsense transcripts 1 using the numbering given in entry NP_002902.2 (GeneID 5976).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UPF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RENT1/UPF1/hUPF1 Antibody

• ATP-dependent helicase RENT1
• EC 3.6.1
• EC 3.6.4.-
• FLJ43809
• HUPF1
• KIAA0221FLJ46894
• Nonsense mRNA reducing factor 1
• NORF1
• NORF1delta helicase
• pNORF1
• regulator of nonsense transcripts 1
• RENT1
• RENT1UP Frameshift 1
• UPF1 regulator of nonsense transcripts homolog (yeast)
• UPF1
• up-frameshift mutation 1 homolog
• Up-frameshift suppressor 1 homolog
• yeast Upf1p homolog

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RENT1/UPF1/hUPF1 Antibody (NBP1-05967)(2)

Publications using NBP1-05967

• Nickless A, Jackson E, Marasa J et al. Intracellular calcium regulates nonsense-mediated mRNA decay. Nat. Med. 2014-07-27 [PMID: 25064126] (WB, Human)
  
  Details:
  UPF2 antibody used for WB in shRNA-mediated UPF2 knockdown U2OS -nonsense-mediated mRNA decay/NMD reporter (multicolored bioluminescence-based reporter system) cells. WB image - Figure 1e.
• Martins R, Proenca D, Silva B et al. Alternative polyadenylation and nonsense-mediated decay coordinately regulate the human HFE mRNA levels. PLoS One 2012-01-01 [PMID: 22530027]

Bioinformatics

• Gene Symbol: UPF1
• Entrez:
  • 5976(Human)
  • 19704 (Mouse)
• Uniprot:
  • Q92900(Human)