Recombinant Human RELM beta Protein Summary
Description |
An un-tagged recombinant protein corresponding to Human RELM beta Source: E. coli Amino Acid Sequence: MQCSLDSVMD KKIKDVLNSL EYSPSPISKK LSCASVKSQG RPSSCPAGMA VTGCACGYGC GSWDVQLETT CHCQCSVVDW TTARCCHLT |
Specificity |
RELM beta is a non-covalent homodimer, 9.5/19.0 kDa (89/178 aa) |
Preparation Method |
Protein is derived from genetically modified E. coli. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
RETNLB |
Purity |
>90%, by SDS-PAGE |
Endotoxin Note |
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method). |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
19 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer |
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) |
Preservative |
No Preservative |
Concentration |
LYOPH |
Purity |
>90%, by SDS-PAGE |
Reconstitution Instructions |
Sterile 0.1% Trifluoroacetic Acid(TFA) at 0.1 mg/mL |
Notes
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.
Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Alternate Names for Recombinant Human RELM beta Protein
Background
RELM beta is a family of resistin-like molecules (RELMs) has been identified in rodents and humans. Resistin is a hormone produced by fat cells. RELMalpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue. Another family member, RELMbeta, is a secreted protein expressed only in the gastrointestinal tract, particularly the colon, in both mouse and human. RELMbeta gene expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation. Resistin and the RELMs share a cysteine composition and other signature features. Thus, the RELMs together with resistin comprise a class of tissue-specific signaling molecules. mRELM-alpha should migrate as a monomer under complete reducing conditions. However, it migrates as dimers, trimers, and even higher order multimers under non-reducing conditions.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is
guaranteed for 1 year from date of receipt.
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