Relaxin-1 Overexpression Lysate (Native)

Images

 
Western Blot: Relaxin-1 Overexpression Lysate (Adult Normal) [NBL1-15385] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Relaxin-1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Relaxin-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203283 and based on accession number NM_006911. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens relaxin 1 (RLN1), mRNA.
Gene
RLN1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Relaxin-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Relaxin-1 Overexpression Lysate (Native)

  • bA12D24.3.1
  • bA12D24.3.2
  • H1
  • preprorelaxin H1
  • prorelaxin H1
  • relaxin 1 (H1)
  • relaxin 1
  • relaxin H1
  • Relaxin1
  • Relaxin-1
  • RLN1
  • RLXH1

Background

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In the human there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3. RLN1 and RLN2 share high sequence homology. This encoded protein is synthesized as a single-chain polypeptide but the active form consists of an A chain and a B chain linked by disulfide bonds; however, their exact cleavage sites have not been described. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. This gene has multiple polyadenylation sites. There are multiple alternatively spliced transcript variants described for this gene but their full length nature is not known yet. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Relaxin-1 Lysate (NBL1-15385) (0)

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Bioinformatics

Gene Symbol RLN1
Entrez