Reg2 Antibody (239237)

Western blot shows lysates of NS0 mouse myeloma cell line, mouse small intestine and pancreas tissue. PVDF membrane was probed with 2 µg/mL of Mouse Reg2 Monoclonal Antibody (Catalog # MAB2098) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 239237
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg2 Antibody (239237) Summary

• Immunogen: E. coli-derived recombinant mouse Reg2
• Specificity: Detects mouse Reg2 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Reg1, recombinant rat (rr) Reg2, rmReg3A, recombinant human Reg4, or rrReg4.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Reg2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg2 Antibody (239237)

• Reg2
• regenerating islet-derived 2
• RegII

Background

Reg2 is a secreted C-type lectin that is expressed in regenerating pancreatic islets and stimulates the growth of pancreatic beta cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg2 Antibody (MAB2098)(2)

Publications using MAB2098

• X Yan, Z Zhao, J Weaver, T Sun, JW Yun, CA Roneker, F Hu, NM Doliba, CCW McCormick, MZ Vatamaniuk, XG Lei Role and mechanism of REG2 depletion in insulin secretion augmented by glutathione peroxidase-1 overproduction Redox Biology, 2022-08-30;56(0):102457. 2022-08-30 [PMID: 36063729] (Immunoprecipitation, Mouse)
• Huszarik K, Wright B, Keller C Adjuvant immunotherapy increases beta cell regenerative factor Reg2 in the pancreas of diabetic mice. J. Immunol., 2010-09-27;185(9):5120-9. 2010-09-27 [PMID: 20876350] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Reg2