Recombinant Rat UNC5H1 Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Rat UNC5H1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H1/Fc Chimera at 5 µg/mL (100 µL/well) can bind rchNetrin-1 with a linear range of 6-400 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived rat UNC5H1 protein
  

  Rat UNC5H1 (Gln26 - Asp358) Accession # O08721	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: O08721
• N-terminal Sequence: No results obtained: Gln26 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Unc5a
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 63.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat UNC5H1 Fc Chimera Protein, CF

• KIAA1976netrin receptor UNC5A
• netrin receptor Unc5h1
• Protein unc-5 homolog 1
• Protein unc-5 homolog A
• unc5 (C.elegans homolog) a
• unc-5 homolog 1
• unc-5 homolog A (C. elegans)
• UNC5A
• UNC5H1
• UNC5H1FLJ16449

Background

UNC5H1, also known as UNC5A, is a member of the UNC5 (Drosophila uncoordinated-5 homology) family of type I transmembrane proteins within the immunoglobulin (Ig) superfamily. UNC5H1-4 (UNC5A-D, respectively) have two Ig and one or two thrombospondin type 1 (TSP1) domains in their extracellular regions, and a ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C‑terminal death domain (DD) in their cytoplasmic regions (1, 2). Rat UNC5H1 cDNA encodes 898 amino acids (aa) including a 25 aa signal peptide, a 336 aa extracellular domain with two TSP1 domains, and a 516 aa cytoplasmic domain. Within the extracellular domain, rat UNC5H1 shares approximately 98% aa sequence identity with mouse and human UNC5H1 (except for the lack of one TSP1 domain in human UNC5H1), and 65% aa identity with rat UNC5H2/UNC5B. UNC5 proteins are receptors for the netrin/UNC6 family of laminin‑related secreted axon guidance cues; UNC5H1 has been shown to bind netrin 1, 3 and 4 (2 ‑ 4). Netrins can act as a chemoattractant for some axons in the presence of DCC (Deleted in Colorectal Cancer) proteins. However, UNC5H1 association with netrins changes them to chemorepellents (1, 5, 6). UNC5H1 is expressed after developmental migration of motor neurons, presumably halting their migration (7). UNC5H1 can be removed from the cell surface via endocytosis following phosphorylation by protein kinase C (PKC) in complex with PICK1. In turn, PKC activity is regulated by the neuronal G protein‑coupled adenosine receptor A2b R (6, 8, 9). The UNC5 and DCC families also act as dependence receptors, and are pro-apoptotic in the absence of netrins (10 ‑ 13). UNC5H1 transcription is enhanced by the tumor suppressor p53, allowing increased apoptosis of tumor cells in the absence of netrin and the presence of NRAGE (13, 14).

1. Moore, S.W. et al. (2007) Adv. Exp. Med. Biol. 621:17.
2. Leonardo, E.D. et al. (1997) Nature 386:833.
3. Wang, H. et al. (1999) J. Neurosci. 19:4938.
4. Qin, S. et al. (2007) Mol. Cell. Neurosci. 34:243.
5. Hong, K. et al. (1999) Cell 97:927.
6. Bartoe, J.L. et al. (2006) J. Neurosci. 26:3192.
7. Barrett, C. and S. Guthrie (2001) Mech. Dev. 106:163.
8. Williams, M.E. et al. (2003) J. Neurosci. 23:11279.
9. McKenna, W.L. et al. (2008) J. Neurochem. 104:1081.
10. Llambi, F. et al. (2001) EMBO J. 20:2715.
11. Thiebault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
12. Williams, M.E. et al. (2006) Nat. Neurosci. 9:996.
13. Miyamoto, Y. et al. (2010) Int. J. Oncol. 36:1253.
14. Williams, M.E. et al. (2003) J. Biol. Chem. 278:17483.

Bioinformatics

• Gene Symbol: Unc5a
• Uniprot:
  • O08721()