Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized at 3 μg/mL on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived rat Nogo-A protein

  Rat Nogo-A (Met1-Val172) Accession # Q9JK11

  IEGRMDP

  Mouse IgG 2A (Glu98-Lys330)

  N-terminus

  C-terminus

• Accession #: Q9JK11
• N-terminal Sequence: Met1
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Rtn4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-76 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Nogo-A Fc Chimera (aa 1-172) Protein, CF

• ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
• NI220
• NogoA
• Nogo-A
• RTN4
• RTN4A

Background

Nogo, so named as a “No-Go” for neurite outgrowth, is a member of the reticulon family of transmembrane proteins, and is also called reticulon 4 (gene name RTN4) (1-4). Reticulons lack N-terminal signal sequences, share a conserved ~200 amino acid (aa) C-terminus that contains two transmembrane domains and an ER-retention motif, and show a punctate intracellular distribution within the endoplasmic reticulum (ER) that is reminescent of a reticulum (1-3). The N-terminus of intracellular (ER) Nogo-A appears to face the cytoplasm (1-3). However, minor amounts of Nogo-A and Nogo-B are found in the plasma membrane with extracellular N-termini (4-6). Full length rat Nogo-A is a 1163 aa protein with a long (~989 aa) N-terminus that includes bioactive regions (aa 59-172 and 544-725), a transmembrane segment, a connecting loop that contains the bioactive Nogo-66 region, a second transmembrane segment, and a short C-terminus (3). The four Nogo isoforms share the Nogo66 segment, Nogo-A and Nogo-B share aa 1-172, and only Nogo-A contains aa 544-725 (1-3). Rat Nogo-A shares 78% and 91% aa sequence identity with human and mouse Nogo-A, respectively. Rat and human Nogo-A/B also share 78% aa sequence identity within aa 1-172 and 98% within the Nogo-66 loop region. Nogo-A is mainly expressed in oligodendrocytes of the central nervous system, but is also reported in fibroblasts, dorsal root ganglion neurons, macrophages and myoblasts (1-8). Nogo-B is mainly expressed in vascular endothelium and smooth muscle throughout the body (1, 4, 6, 9). The Nogo66 region binds the GPI-linked Nogo receptor/p75 complex on axons, inducing growth cone collapse (5, 7, 10, 11). Either aa 59-172 or 544-725 segments can block neurite outgrowth and fibroblast spreading (5, 6). Nogo-A/B aa 1-172 is also reported to regulate vascular remodeling through binding the Nogo-B receptor (NgBR/NUS1) on vascular cells, and to inhibit neuronal differentiation and promote glial formation from neural progenitors (4, 5, 8, 9, 12).

1. Oertle, T. and M.E. Schwab (2003) Trends Cell Biol. 13:187.
2. GrandPre, T. et al. (2000) Nature 403:439.
3. Chen, M.S. et al. (2000) Nature 403:434.
4. Acevedo, L. et al. (2004) Nat. Med. 10:382.
5. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
6. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
7. Wang, X. et al. (2002) J. Neurosci. 22:5505.
8. Schwab, M.E. (2010) Nat. Rev. Neurosci. 11:799.
9. Miao, R.Q. et al. (2006) Proc. Natl. Acad. Sci. USA 103:10997.
10. Fournier, A.E. et al. (2001) Nature 409:341.
11. Wang, K.C. et al. (2002) Nature 420:74.
12. Guo, Y. et al. (2009) Neurosci. Lett. 458:132.

Publications for Nogo-A (3098-NG)(1)

Publication using 3098-NG

• Sun X, Zhou Z, Fink D, Mata M HspB1 silences translation of PDZ-RhoGEF by enhancing miR-20a and miR-128 expression to promote neurite extension. Mol Cell Neurosci, 2013-10-16;57(0):111-9. 2013-10-16 [PMID: 24141048] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Rtn4
• Uniprot:
  • Q9JK11()