Recombinant Rat Jagged 1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Rat Jagged 1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Jagged 1 protein
Rat Jagged 1
Ser32-Asp1067
with substitutions GGARN56-60AE and DR63-64TLVRPY
Accession # Q63722.2
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Jag1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
141.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
180 kDa, reducing conditions
Publications
Read Publications using
599-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Jagged 1 Fc Chimera Protein, CF

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Rat Jagged1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged1 shows 98% and 99% aa identity to human and mouse Jagged1 extracellular domains respectively. Relative to the extracellular region of rat Jagged2, the aa identity is 58%.

  1. Lindsell, C.E. et al. (1995) Cell 80:909.
  2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.

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Publications for Jagged 1 (599-JG)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Enzyme Assay Substrate, In Vivo.


Filter By Application
Bioassay
(14)
Enzyme Assay Substrate
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(7)
Rat
(6)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 599-JG Applications Species
KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling Elife, 2018;7(0):. 2018 [PMID: 30589410] (Bioassay, Mouse) Bioassay Mouse
Y He, M Zhang, Y Wu, H Jiang, H Fu, Y Cai, Z Xu, C Liu, B Chen, T Yang Aberrant activation of Notch-1 signaling inhibits podocyte restoration after islet transplantation in a rat model of diabetic nephropathy Cell Death Dis, 2018;9(10):950. 2018 [PMID: 30237561] (Bioassay, Mouse) Bioassay Mouse
J Davis-Know, JE Turner, A Turner, S Damian-Lor, N Hagler, T Henderson, IF Emery, K Bond, CW Duarte, CPH Vary, J Eldrup-Jor, L Liaw Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling Lab. Invest., 2018;0(0):. 2018 [PMID: 29795127] (Bioassay, Rat) Bioassay Rat
S Varshney, P Stanley Notch Ligand Binding Assay Using Flow Cytometry Bio Protoc, 2017;7(23):. 2017 [PMID: 29333477] (Bioassay, Human) Bioassay Human
Varshney S, Stanley P Notch Ligand Binding Assay Using Flow Cytometry. Bio Protoc, 0;7(23):. 0 [PMID: 29333477] (Bioassay, Rat) Bioassay Rat
D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren Selective regulation of Notch ligands during angiogenesis is mediated by vimentin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533359] (Bioassay, Mouse) Bioassay Mouse
Yi Ji Jagged1/Notch3 Signaling Modulates Hemangioma-Derived Pericyte Proliferation and Maturation Cell. Physiol. Biochem, 2016;40(5):895-907. 2016 [PMID: 27941324] (Bioassay, Rat) Bioassay Rat
Kim K, Chen C, Alpini G, Lau L CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB. J Clin Invest, 2015;125(5):1886-900. 2015 [PMID: 25822023] (In Vivo, Mouse) In Vivo Mouse
Chen T, Margariti A, Kelaini S, Cochrane A, Guha S, Hu Y, Stitt A, Zhang L, Xu Q MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling. Stem Cells, 2015;33(5):1405-18. 2015 [PMID: 25535084] (Bioassay, Mouse) Bioassay Mouse
He W, Hu J, Xia Y, Yan R beta-site amyloid precursor protein cleaving enzyme 1(BACE1) regulates Notch signaling by controlling the cleavage of Jagged 1 (Jag1) and Jagged 2 (Jag2) proteins. J Biol Chem, 2014;289(30):20630-7. 2014 [PMID: 24907271] (Enzyme Assay Substrate, Rat) Enzyme Assay Substrate Rat
Show All 18 Publications.

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Bioinformatics

Gene Symbol Jag1
Uniprot