Recombinant Rat Jagged 1 Fc Chimera Protein, CF

Images

 
There are currently no images for Jagged 1 (599-JG).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Rat Jagged 1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Jagged 1 protein
Rat Jagged 1
Ser32-Asp1067
with substitutions GGARN56-60AE and DR63-64TLVRPY
Accession # Q63722.2
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser32
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Jag1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
141.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
180 kDa, reducing conditions
Publications
Read Publications using
599-JG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Jagged 1 Fc Chimera Protein, CF

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Rat Jagged1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged1 shows 98% and 99% aa identity to human and mouse Jagged1 extracellular domains respectively. Relative to the extracellular region of rat Jagged2, the aa identity is 58%.

  1. Lindsell, C.E. et al. (1995) Cell 80:909.
  2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.

Customers Who Viewed This Item Also Viewed...

NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-78486
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
MAB208
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF1190
Species: Rt
Applications: Block, CyTOF-ready, Flow, IHC, WB
210-TA
Species: Hu
Applications: BA
NB100-689
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB100-56098
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-29617
Species: Hu
Applications: IHC, IHC-Fr, IHC-P, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB100-93551
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-28566
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
NBP2-46085
Species: Hu
Applications: IHC, WB

Publications for Jagged 1 (599-JG)(29)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 4 applications: Bioassay, Cell Culture, Enzyme Assay Substrate, In Vivo.


Filter By Application
Bioassay
(24)
Cell Culture
(1)
Enzyme Assay Substrate
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(8)
Mouse
(10)
Rat
(8)
Hamster
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using 599-JG Applications Species
JH Won, JS Choi, JI Jun CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation Nature Communications, 2022;13(1):3117. 2022 [PMID: 35660741] (Bioassay, Mouse) Bioassay Mouse
Y Cheng, W Gu, G Zhang, X Guo Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure BMC pulmonary medicine, 2022;22(1):139. 2022 [PMID: 35410206] (Bioassay, Rat) Bioassay Rat
S Zhang, A Miyakawa, M Wickström, C Dyberg, L Louhivuori, M Varas-Godo, K Kemppainen, S Kanatani, D Kaczynska, ID Ellström, L Elfman, P Kronqvist, H Repo, K Mikoshiba, C Sahlgren, JI Johnsen, P Uhlén GIT1 protects against breast cancer growth through negative regulation of Notch Nature Communications, 2022;13(1):1537. 2022 [PMID: 35318302] (Bioassay, Human) Bioassay Human
J Meng, YZ Jiang, S Zhao, Y Tao, T Zhang, X Wang, Y Zhang, K Sun, M Yuan, J Chen, Y Wei, X Lan, M Chen, CJ David, Z Chang, X Guo, D Pan, M Chen, ZM Shao, Y Kang, H Zheng Tumor-derived Jagged1 promotes cancer progression through immune evasion Cell Reports, 2022;38(10):110492. 2022 [PMID: 35263601] (Bioassay, Mouse) Bioassay Mouse
B Liu, D Wang, T Xiong, Y Liu, X Jing, J Du, Q She Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes Stem Cells International, 2021;2021(0):8859071. 2021 [PMID: 33897781] (Bioassay, Mouse) Bioassay Mouse
S González, M Halabi, D Ju, M Tsai, SX Deng Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium Cells, 2020;9(9):. 2020 [PMID: 32842657] (Bioassay, Human) Bioassay Human
S González, M Halabi, D Ju, M Tsai, SX Deng Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium Cells, 2020;9(9):. 2020 [PMID: 32842657] (Bioassay, Human) Bioassay Human
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32820046] (Bioassay, Hamster) Bioassay Hamster
S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32820046] (Bioassay, Hamster) Bioassay Hamster
WC Chung, Y Zhou, A Atfi, K Xu Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia Neoplasia, 2019;21(8):810-821. 2019 [PMID: 31276933] (Bioassay, Human) Bioassay Human
Show All 29 Publications.

Reviews for Jagged 1 (599-JG) (0)

There are no reviews for Jagged 1 (599-JG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Jagged 1 (599-JG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat Jagged 1 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Jag1
Uniprot