Recombinant Rat Jagged 1 Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Jagged 1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED 50 for this effect is 0.5-2.0 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived rat Jagged 1 protein

  Rat Jagged 1 Ser32-Asp1067 with substitutions GGARN56-60AE and DR63-64TLVRPY Accession # Q63722.2

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  6-His tag

  N-terminus

  C-terminus

• Accession #: Q63722.2
• N-terminal Sequence: Ser32
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Jag1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 141.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 180 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Jagged 1 Fc Chimera Protein, CF

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Rat Jagged1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged1 shows 98% and 99% aa identity to human and mouse Jagged1 extracellular domains respectively. Relative to the extracellular region of rat Jagged2, the aa identity is 58%.

1. Lindsell, C.E. et al. (1995) Cell 80:909.
2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.

Publications for Jagged 1 (599-JG)(28)

Publications using 599-JG

• Thambyrajah, R;Maqueda, M;Neo, WH;Imbach, K;Guillén, Y;Grases, D;Fadlullah, Z;Gambera, S;Matteini, F;Wang, X;Calero-Nieto, FJ;Esteller, M;Florian, MC;Porta, E;Benedito, R;Göttgens, B;Lacaud, G;Espinosa, L;Bigas, A; Cis inhibition of NOTCH1 through JAGGED1 sustains embryonic hematopoietic stem cell fate Nature communications 2024-02-21 [PMID: 38383534] (Bioassay, Mouse)
• Chen, Y;Liao, G;Ma, T;Li, L;Yang, J;Shen, B;Lu, Y;Si, H; YY1/miR-140-5p/Jagged1/Notch axis mediates cartilage progenitor/stem cells fate reprogramming in knee osteoarthritis International immunopharmacology 2023-06-07 [PMID: 37295026] (In Vivo, Rat)
• JH Won, JS Choi, JI Jun CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation Nature Communications, 2022-06-03;13(1):3117. 2022-06-03 [PMID: 35660741] (Bioassay, Mouse)
• Y Cheng, W Gu, G Zhang, X Guo Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure BMC pulmonary medicine, 2022-04-11;22(1):139. 2022-04-11 [PMID: 35410206] (Bioassay, Rat)
• S Zhang, A Miyakawa, M Wickström, C Dyberg, L Louhivuori, M Varas-Godo, K Kemppainen, S Kanatani, D Kaczynska, ID Ellström, L Elfman, P Kronqvist, H Repo, K Mikoshiba, C Sahlgren, JI Johnsen, P Uhlén GIT1 protects against breast cancer growth through negative regulation of Notch Nature Communications, 2022-03-22;13(1):1537. 2022-03-22 [PMID: 35318302] (Bioassay, Human)
• J Meng, YZ Jiang, S Zhao, Y Tao, T Zhang, X Wang, Y Zhang, K Sun, M Yuan, J Chen, Y Wei, X Lan, M Chen, CJ David, Z Chang, X Guo, D Pan, M Chen, ZM Shao, Y Kang, H Zheng Tumor-derived Jagged1 promotes cancer progression through immune evasion Cell Reports, 2022-03-08;38(10):110492. 2022-03-08 [PMID: 35263601] (Bioassay, Mouse)
• B Liu, D Wang, T Xiong, Y Liu, X Jing, J Du, Q She Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes Stem Cells International, 2021-04-09;2021(0):8859071. 2021-04-09 [PMID: 33897781] (Bioassay, Mouse)
• S González, M Halabi, D Ju, M Tsai, SX Deng Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium Cells, 2020-08-22;9(9):. 2020-08-22 [PMID: 32842657] (Bioassay, Human)
• S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Bioassay, Mouse)
• WC Chung, Y Zhou, A Atfi, K Xu Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia Neoplasia, 2019-07-02;21(8):810-821. 2019-07-02 [PMID: 31276933] (Bioassay, Human)
• WR Lan, S Pan, HY Li, C Sun, X Chang, K Lu, CQ Jiang, R Zuo, Y Zhou, CQ Li Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats Stem Cells Int, 2019-05-06;2019(0):8404168. 2019-05-06 [PMID: 31249601] (Cell Culture, Rat)
• KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling Elife, 2018-12-27;7(0):. 2018-12-27 [PMID: 30589410] (Bioassay, Mouse)
• Y He, M Zhang, Y Wu, H Jiang, H Fu, Y Cai, Z Xu, C Liu, B Chen, T Yang Aberrant activation of Notch-1 signaling inhibits podocyte restoration after islet transplantation in a rat model of diabetic nephropathy Cell Death Dis, 2018-09-20;9(10):950. 2018-09-20 [PMID: 30237561] (Bioassay, Mouse)
• J Davis-Know, JE Turner, A Turner, S Damian-Lor, N Hagler, T Henderson, IF Emery, K Bond, CW Duarte, CPH Vary, J Eldrup-Jor, L Liaw Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling Lab. Invest., 2018-05-23;0(0):. 2018-05-23 [PMID: 29795127] (Bioassay, Rat)
• S Varshney, P Stanley Notch Ligand Binding Assay Using Flow Cytometry Bio Protoc, 2017-12-05;7(23):. 2017-12-05 [PMID: 29333477] (Bioassay, Human, Rat)
• D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren Selective regulation of Notch ligands during angiogenesis is mediated by vimentin Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533359] (Bioassay, Mouse)
• Yi Ji Jagged1/Notch3 Signaling Modulates Hemangioma-Derived Pericyte Proliferation and Maturation Cell. Physiol. Biochem, 2016-12-07;40(5):895-907. 2016-12-07 [PMID: 27941324] (Bioassay, Rat)
• Kim K, Chen C, Alpini G, Lau L CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB. J Clin Invest, 2015-03-30;125(5):1886-900. 2015-03-30 [PMID: 25822023] (In Vivo, Mouse)
• Chen T, Margariti A, Kelaini S, Cochrane A, Guha S, Hu Y, Stitt A, Zhang L, Xu Q MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling. Stem Cells, 2015-05-01;33(5):1405-18. 2015-05-01 [PMID: 25535084] (Bioassay, Mouse)
• He W, Hu J, Xia Y, Yan R beta-site amyloid precursor protein cleaving enzyme 1(BACE1) regulates Notch signaling by controlling the cleavage of Jagged 1 (Jag1) and Jagged 2 (Jag2) proteins. J Biol Chem, 2014-07-25;289(30):20630-7. 2014-07-25 [PMID: 24907271] (Enzyme Assay Substrate, Rat)
• Sjoqvist M, Antfolk D, Ferraris S, Rraklli V, Haga C, Antila C, Mutvei A, Imanishi S, Holmberg J, Jin S, Eriksson J, Lendahl U, Sahlgren C PKCzeta regulates Notch receptor routing and activity in a Notch signaling-dependent manner. Cell Res, 2014-03-25;24(4):433-50. 2014-03-25 [PMID: 24662486] (Bioassay, Human)
• Boucher , Joshua M, Harrington , Anne, Rostama , Bahman, Lindner , Volkhard, Liaw , Lucy A receptor-specific function for Notch2 in mediating vascular smooth muscle cell growth arrest through cyclin-dependent kinase inhibitor 1B. Circ Res, 2013-08-21;113(8):975-85. 2013-08-21 [PMID: 23965337] (Bioassay, Human)
• Sun F, Mao X, Xie L, Ding M, Shao B, Jin K Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia. Aging Cell, 2013-08-06;12(6):978-87. 2013-08-06 [PMID: 23834718] (Bioassay, Rat)
• Benner , Eric J, Luciano , Dominic, Jo , Rebecca, Abdi , Khadar, Paez-Gonzalez , Patricia, Sheng , Huaxin, Warner , David S, Liu , Chunlei, Eroglu , Cagla, Kuo , Chay T Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013-04-24;497(7449):369-73. 2013-04-24 [PMID: 23615612] (Bioassay, Mouse)
• Oya S, Yoshikawa G, Takai K, Tanaka JI, Higashiyama S, Saito N, Kirino T, Kawahara N Attenuation of Notch signaling promotes the differentiation of neural progenitors into neurons in the hippocampal CA1 region after ischemic injury. Neuroscience, 2008-10-31;158(2):683-92. 2008-10-31 [PMID: 19017538] (In Vivo, Rat)
• Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage. Genes Dev., 2008-01-15;22(2):152-65. 2008-01-15 [PMID: 18198334] (Bioassay, Human)
• Santos MA, Sarmento LM, Rebelo M, Doce AA, Maillard I, Dumortier A, Neves H, Radtke F, Pear WS, Parreira L, Demengeot J Notch1 engagement by Delta-like-1 promotes differentiation of B lymphocytes to antibody-secreting cells. Proc. Natl. Acad. Sci. U.S.A., 2007-09-18;104(39):15454-9. 2007-09-18 [PMID: 17878313] (Bioassay, Mouse)
• Salero E, Hatten ME Differentiation of ES cells into cerebellar neurons. Proc. Natl. Acad. Sci. U.S.A., 2007-02-09;104(8):2997-3002. 2007-02-09 [PMID: 17293457] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Jag1
• Uniprot:
  • Q63722.2()