Recombinant Rat CD155/PVR His-tag Protein, CF

When Recombinant Rat CD155/PVR (Catalog # 9764‑CD) is immobilized at 1 μg/mL,100 μL/well, Recombinant Mouse CD96 (Catalog # 5690‑CD) binds with an ED50 of 1.5‑7.5 ng/mL.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat CD155/PVR His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Rat CD155/PVR is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD96 (Catalog # 5690-CD) that produces 50% optimal binding response is 1.5-7.5 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived rat CD155/PVR protein
  Glu34-Leu351, with a C-terminal 6-His tag
• Accession #: NP_058772
• N-terminal Sequence: Glu34
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-71 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CD155/PVR His-tag Protein, CF

• CD155 antigen
• CD155
• HVED
• NECL5
• Necl-5
• nectin-like 5
• Nectin-like protein 5
• poliovirus receptor
• PVR
• PVS
• PVSFLJ25946
• Tage4

Background

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). The protein may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3, 4). Mature rat CD155/PVR consists of three Ig-like domains and a 21-amino acid (aa) transmembrane segment. Within the ECD, rat CD155/PVR shares 44% and 73% aa sequence identity with human and mouse CD155/PVR, respectively. CD155/PVR is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155/PVR is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155/PVR expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM-1 to endothelial cell PVR promotes transendothelial migration (7).

1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
3. Sloan, K. et al. (2004) BMC Cancer. 4:73.
4. Sato, T. et al. (2004) Genes to Cells 9:791.
5. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
6. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
8. Maier, M.K. et al. (2007) Eur. J. Immunol. 37:2214.
9. Lozano, E. et al. (2013) J. Immunol. 191:3673.
10. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
11. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
12. Chan, C.J. et al. (2010) J. Immunol. 184:902.

Bioinformatics

• Uniprot:
  • NP_058772()