Recombinant Rat Agrin Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat Agrin Protein, CF Summary

Details of Functionality
Measured by its ability to induce acetylcholine receptor clustering on myotubes differentiated from C2C12 mouse myoblast cells. Ferns, M.J. et al. (1993) Neuron 11:491. The ED50 for this effect is 2-6 ng/mL. Measured by its binding ability in a functional ELISA. When recombinant human LRP-4 is coated at 0.5 μg/mL, Recombinant Rat Agrin binds with an apparent Kd <3 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat Agrin protein
Ala1153-Pro1959 (Pro1788-Ser1798 del), with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Agrn
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100 kDa, reducing conditions
Publications
Read Publications using
550-AG/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Agrin Protein, CF

  • agrin proteoglycan
  • Agrin
  • AGRN

Background

Agrin is a 400‑600 kDa heparan sulfate proteoglycan component of the extracellular matrix. The N‑terminal half of rat Agrin, which mediates ECM interactions, contains nine Kazal-type protease inhibitor domains, two Laminin EGF-like domains, and one SEA domain. The C-terminal half contains four EGF-like repeats and three Laminin globular G domains. Human Agrin also contains a Laminin‑binding N-terminal Agrin domain (NtA), and mouse and chick Agrin include the NtA domain only by the use of an alternate promoter. Additional isoforms are generated by alternate splicing at sites Y and Z in the C-terminal half of rat Agrin (known as A and B, respectively in chick). Agrin isoforms that contain an insert at site Z (Z+ forms) are known as neural Agrin and are selectively produced by motoneurons. Other isoforms are known as muscle Agrin and are additionally expressed in non-neuronal tissues, particularly in basement membranes of the lung and kidney (1-3). This recombinant protein consists of the C-terminal half of rat Agrin and contains a nine amino acid (aa) insert at the Z site. It shares 59%, 80%, and 94% aa sequence identity with comparable regions of chick, human, and mouse Agrin, respectively. The C-terminal half of Z- and Z+ Agrin binds to alpha -Dystroglycan and mediates adhesion between motoneurons and myotubes at the neuromuscular junction (NMJ) (4-6). In contrast, only Z+ Agrin is effective at inducing clustering of the postsynaptic Acetylcholine Receptor (AChR) and presynaptic motoneuron differentiation (7, 8). Agrin‑induced AChR clustering requires a myotube receptor complex that contains alpha -Dystroglycan, MuSK, and LRP4 (4, 9-11). Agrin exhibits many functions in addition to NMJ development. It is enriched in senile Alzheimer’s disease plaques where it binds the A beta (1-40) peptide and promotes amyloid fibril formation (12). It regulates neuronal excitability by binding and inhibiting the alpha 3 subunit of the neuronal Na/K ATPase (13). It functions as an epithelial cell attachment receptor for HIV-1 through interactions with the gp41 coat protein (14). During T cell activation, Agrin contributes to formation of the immunological synapse and regulates the threshold of T cell activation (15).
  1. Jury, E.C. and P.S. Kabouridis (2010) Arthritis Res. Ther. 12:205.
  2. Bezakova, G. and M.A. Ruegg (2003) Nat. Rev. Mol. Cell Biol. 4:295.
  3. Rupp, F. et al. (1991) Neuron 6:811.
  4. Gee, S.H. et al. (1994) Cell 77:675.
  5. Sugiyama, J. et al. (1994) Neuron 13:103.
  6. Gesemann, M. et al. (1998) J. Biol. Chem. 273:600.
  7. Burgess, R.W. et al. (1999) Neuron 23:33.
  8. Ferns, M.J. et al. (1993) Neuron 11:491.
  9. Glass, D.J. et al. (1996) Cell 85:513.
  10. Kim, N. et al. (2008) Cell 135:334.
  11. Zhang, B. et al. (2008) Neuron 60:285.
  12. Cotman, S.L. et al. (2000) Mol. Cell. Neurosci. 15:183.
  13. Hilgenberg, L.G.W. et al. (2006) Cell 125:359.
  14. Alfsen, A. et al. (2005) Mol. Biol. Cell 16:4267.
  15. Khan, A.A. et al. (2001) Science 292:1681.

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Publications for Agrin (550-AG/CF)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 4 applications: Bioassay, Cell Culture, Differentiation, In Vivo.


Filter By Application
Bioassay
(20)
Cell Culture
(1)
Differentiation
(2)
In Vivo
(1)
All Applications
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Human
(4)
Mouse
(14)
Rat
(1)
Xenopus
(2)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 550-AG/CF Applications Species
K Gros, U Matkovi?, G Parato, K Miš, E Luin, A Bernareggi, M Sciancalep, T Marš, P Lorenzon, S Pirkmajer Neuronal Agrin Promotes Proliferation of Primary Human Myoblasts in an Age-Dependent Manner International Journal of Molecular Sciences, 2022-10-04;23(19):. 2022-10-04 [PMID: 36233091] (Cell Culture, Human) Cell Culture Human
I Klockner, C Schutt, T Gerhardt, T Boettger, T Braun Control of CRK-RAC1 activity by the miR-1/206/133 miRNA family is essential for neuromuscular junction function Nature Communications, 2022-06-08;13(1):3180. 2022-06-08 [PMID: 35676269] (Bioassay, Mouse) Bioassay Mouse
OA Shipton, CS Tang, O Paulsen, M Vargas-Cab Differential vulnerability of hippocampal CA3-CA1 synapses to Abeta Acta neuropathologica communications, 2022-04-04;10(1):45. 2022-04-04 [PMID: 35379353] (Differentiation, Differentiation, Human) Differentiation, Differentiation Human
T Yamamotoya, S Hasei, Y Akasaka, Y Ohata, Y Nakatsu, M Kanna, M Fujishiro, H Sakoda, H Ono, A Kushiyama, H Misawa, T Asano Involvement of neuronal and muscular Trk-fused gene (TFG) defects in the development of neurodegenerative diseases Scientific Reports, 2022-02-04;12(1):1966. 2022-02-04 [PMID: 35121777] (Bioassay, Mouse) Bioassay Mouse
C Pinto, V Pérez, J Mella, M Albistur, T Caprile, FC Bronfman, JP Henríquez Transport and Secretion of the Wnt3 Ligand by Motor Neuron-like Cells and Developing Motor Neurons Biomolecules, 2021-12-17;11(12):. 2021-12-17 [PMID: 34944540] (Bioassay, Mouse) Bioassay Mouse
J Oury, W Zhang, N Leloup, A Koide, AD Corrado, G Ketavarapu, T Hattori, S Koide, SJ Burden Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia Nature, 2021-06-23;0(0):. 2021-06-23 [PMID: 34163073] (Bioassay, Mouse) Bioassay Mouse
D Proietti, L Giordani, M De Bardi, C D'Ercole, B Lozanoska-, S Amadio, C Volontè, S Marinelli, A Muchir, M Bouchè, G Borsellino, A Sacco, PL Puri, L Madaro Activation of skeletal muscle-resident glial cells upon nerve injury JCI Insight, 2021-04-08;0(0):. 2021-04-08 [PMID: 33661767] (Bioassay, Mouse) Bioassay Mouse
MJ Oentaryo, AC Tse, CW Lee Neuronal MT1-MMP mediates ECM clearance and Lrp4 cleavage for agrin deposition and signaling in presynaptic development J. Cell. Sci., 2020-08-05;0(0):. 2020-08-05 [PMID: 32591486] (Bioassay, Xenopus) Bioassay Xenopus
PJ Chen, D Zelada, DC Belhasan, M Akaaboune Phosphorylation of alpha-dystrobrevin is essential for alphakap accumulation and acetylcholine receptor stability J. Biol. Chem., 2020-06-12;0(0):. 2020-06-12 [PMID: 32532815] (Bioassay, Mouse) Bioassay Mouse
G Xing, H Jing, L Zhang, Y Cao, L Li, K Zhao, Z Dong, W Chen, H Wang, R Cao, WC Xiong, L Mei A mechanism in Agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome Elife, 2019-09-24;8(0):. 2019-09-24 [PMID: 31549961] (Bioassay, Mouse) Bioassay Mouse
Show All 22 Publications.

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Bioinformatics

Gene Symbol Agrn
Uniprot