Recombinant Mouse Wnt-5b Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Wnt-5b Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-5b protein
  Gln31-Lys372
• Accession #: AAH10775
• N-terminal Sequence: No results obtained: Gln31 predicted
• Protein/Peptide Type: Recombinant Proteins
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-5b Protein, CF

• MGC2648
• protein Wnt-5b
• wingless-type MMTV integration site family, member 5B
• WNT-5B protein
• Wnt5b
• Wnt-5b

Background

Wnt proteins are cysteine-rich secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1-3). Downstream effects of Wnt signaling occur through multiple pathways with differing intracellular components: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca²⁺ pathway, and the planar cell polarity (PCP) pathway (1-4). Wnt-5b is a 49 kDa glycoprotein that is implicated in the Wnt/Ca²⁺ and PCP pathways (4‑8). These pathways can inhibit canonical Wnt/ beta -catenin signaling (4, 7). Human and mouse Wnt-5b are synthesized as 359 amino acid (aa) precursors that contain a 17 aa signal sequence and a 342 aa mature region. Mature mouse Wnt-5b shares 94%, 98%, 90% and 88% aa identity with mature human, rat, chick and Xenopus Wnt-5b, respectively. Although Wnt-5a and Wnt-5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt-5a, promotes cell cycle progression and is weakly transforming (8, 9). Wnt-5a and -5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone (5, 6, 10). In contrast to more focused expression of Wnt-5a, Wnt-5b is constitutively expressed at low levels throughout mouse embryonic development (10, 11). In adult mice, Wnt-5b is widely expressed, including heart, liver, brain, lung, testes, kidney, and pancreas (11-13). Wnt-5b is up‑regulated during early adipogenesis, and its overexpression in 3T3-L1 cells partially inhibits canonical Wnt suppression of adipogenesis (7, 14). It enhances PPAR gamma expression and promotes differentiation of preadipocytes (14). Human Wnt-5b polymorphisms have been associated with Type II diabetes (12).

1. Mikels, A.J. & R. Nusse, 2006, Oncogene 25:7461.
2. Miller, J.R. (2002) Genome Biol. 3:3001.
3. Li, L et al., 2004, J. Neurosci 29:5873.
4. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
5. Fazzi, R. et al. (2011) PLoS ONE 6:e25600.
6. Bradley, E.W. and M.H. Drissi (2011) J. Cell. Physiol. 226:1683.
7. Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
8. Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
9. Yang, Y. et al. (2003) Development 130:1003.
10. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
11. Gavin, B. J. et al. (1990) Genes Dev. 4:2319.
12. Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
13. Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
14. van Tienen, F.H.J. et al. (2009) Biochem. Biophys. Res. Commun. 387:207.

Publications for Wnt-5b (3006-WN/CF)(4)

Publications using 3006-WN/CF

• X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 2019-09-25;8(10):. 2019-09-25 [PMID: 31557955] (Bioassay, Mouse)
• K Kessenbroc, P Smith, SC Steenbeek, N Pervolarak, R Kumar, Y Minami, A Goga, L Hinck, Z Werb Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;0(0):. 2017-03-07 [PMID: 28270600] (Bioassay, Mouse)
• Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 2013-11-08;385(2):291-303. 2013-11-08 [PMID: 24211762] (Bioassay, Rat)
• Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J. Biol. Chem., 2010-01-21;285(12):9172-9. 2010-01-21 [PMID: 20093360] (Binding Assay, Human)

Bioinformatics

• Uniprot:
  • AAH10775()