Recombinant Mouse THSD1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse THSD1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 10⁴ cells/well are added to rmTHSD1 coated plates (30 µg/mL, 100 µL/well), approximately 50-80% will adhere after 60 minutes at 37° C.
• Source: Mouse myeloma cell line, NS0-derived mouse THSD1 protein
  Glu25-Asn412, with a C-terminal 6-His tag
• Accession #: Q9JM61
• N-terminal Sequence: Glu25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Thsd1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-78 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse THSD1 Protein, CF

• thrombospondin type-1 domain-containing protein 1
• thrombospondin, type I, domain 1
• thrombospondin, type I, domain containing 1,4833423O18Rik
• THSD1
• TMTSP
• TMTSPMGC74971
• Transmembrane molecule with thrombospondin module
• UNQ3010

Background

Mouse THSD1 (Thrombospondin type-1 domain-containing protein 1), also known as Transmembrane molecule with thrombospondin module (Tmtsp), is a 95 kDa, type I transmembrane protein (1). It is synthesized as a precursor that is 851 amino acids (aa) in length, and has a 24 aa signal sequence, a 388 aa extracellular region, a 21 aa transmembrane region, and a 418 aa cytoplasmic region. The extracellular region contains six potential N-linked glycosylation sites and the thrombospondin type-1 (TSP1) domain, which is implicated in cell adhesion and migration (1). Also present in the molecule are 9 PKC-phosphorylation sites, 3 Ig-like domains, and a tyrosine-phosphorylation site by its C-terminus (1). Mouse THSD1 shares 78% aa sequence identity with human THSD1. THSD1 is highly expressed in hematopoietic stem cells (HSCs) and progenitor cells, including CD34^-/lowKit⁺Sca-1⁺Lin^- HSCs, CD34⁺Kit⁺Sca-1⁺Lin^- and Lin^- progenitor cells, and to a lesser degree in thymic CD4^-CD8^- cells (1). Expression gradually decreases during T cell differentiation (1). In addition, THSD1 is widely expressed on endothelial cells, with highest expression in the lung (1). THSD1’s ligand and function are unknown, but it is postulated that THSD1 may be involved in the regulation of vasculogenesis and/or angiogenesis through its interaction with its specific ligand (1). THSD1 may function in primitive hematopoietic cells in the bone marrow niche, and through its TSP1 and Ig-like domains, it may play a role in HSC homing to the niche following cell-to-cell contact to maintain hematopoietic stem cell quiescence (1).

1. Takayanagi, S. et al. (2006) Blood 107:4317.

Bioinformatics

• Gene Symbol: Thsd1
• Uniprot:
  • Q9JM61()