Recombinant Mouse SLITRK5 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SLITRK5 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-6 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse SLITRK5 protein
  Ile41-Ser664, with a C-terminal 6-His tag
• Accession #: Q810B7
• N-terminal Sequence: Ile41
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 96-113 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SLITRK5 Protein, CF

• bA364G4.2
• FLJ58374
• KIAA0918
• leucine-rich repeat-containing protein 11
• LRRC11
• SLIT and NTRK-like family, member 5
• SLIT and NTRK-like protein 5
• SLITRK5

Background

SLITRK5 (Slit and Trk-like family member 5; also known as LRR-containing protein 11) is a member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and a Trk-like cytoplasmic region (3). Mouse SLITRK5 is a type I transmembrane (TM) protein that is 957 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 272 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in SLITs. Each LRD  contains seven and eight leucine-rich repeats (LRRs), respectively. Mouse SLITRK5 shares 97% and 99% aa identity with Human and Rat SLITRK5, respectively. The exact function of the LRRs is not known. They are assumed to mediate protein-protein-interactions (4). The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr833 and Tyr917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus (3). SLITRK5 was also shown to be expressed in hematopoietic stem cells, leukemias, and subsets of endothelial cells (5). Loss of SLITRK5 leads to manifestation of OCD-like behaviors in mice. SLITRK-5 was identified as an essential molecule at corticostriatal synapses (6). More recent studies have shown that SLITRK5 mediates BDNF-dependent biological functions through direct interaction with TrkB receptors. SLITRK5 primarily interacts with a trans-synaptic binding partner, protein tyrosine phosphatase δ (PTPδ), but upon BDNF stimulation it shifts to cis-interactions with TrkB (7).

1. Aruga, J. et al. (2003) Gene 315:87.
2. Nagase, T. et al. (2001) DNA Res. 8:179.
3. Aruga, J. & K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
4. Enkhbayar, P. et al. (2003) Proteins 54:394.
5. Milde T. (2007) Leukemia. 21:824.
6. Shmelkov S.V. (2010) Nat. Med. 16:598.
7. Song M. (2015) Dev. Cell. 33:690.

Bioinformatics

• Uniprot:
  • Q810B7()