Recombinant Mouse Semaphorin 6C Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 6C Protein, CF Summary

• Details of Functionality: Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. Concentration of 2.5-10 μg/mL in the presence of 20 ng/mL of rhNT-3 (R&D Systems, Catalog # 267-N3) is sufficient to cause significant growth cone collapse.
  Optimal concentrations should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 6C protein
  Ala26-Ile602, with a C-terminal 10-His tag
• Accession #: Q9WTM3
• N-terminal Sequence: Ala26
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sema6c
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 64.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 74 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 6C Protein, CF

• KIAA1869
• m-SemaY
• m-SemaY2
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C
• Sema Y
• Semaphorin 6C
• semaphorin-6C
• semaphorin-Y
• SEMAY

Background

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 - 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that are most like Class 1 invertebrate semaphorins in structure, and exhibit neuropilin-independent binding to specific plexin A receptors (1 - 3). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53 - 64% within the Sema domain. Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Mouse Sema6C cDNA encodes 931 aa, including a 25 aa signal sequence, a 580 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 305 aa cytoplasmic portion. Alternate exon splicing creates a 923 aa short form (Sema6C.3) that is lacking aa 185 - 224 within the Sema domain, but contains 32 unique aa inserted at aa 586; postnatally, this form predominates in muscle (2, 3). The long form predominates in brain, especially in areas of increased plasticity (4). Mouse Sema6C ECD shares 98%, 92%, 92%, 92% and 88% aa identity with corresponding rat, human, porcine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is downregulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
3. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
4. Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
5. Yoshida, Y. et al. (2006) Neuron 52:775.
6. Svensson, A. et al. (2008) J. Mol. Hist. 39:5.

Bioinformatics

• Gene Symbol: Sema6c
• Uniprot:
  • Q9WTM3()