Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

2 μg/lane of Recombinant Mouse Semaphorin 4B Fc Chimera (Catalog # 10263-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-117 kDa and 200-230 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 4B protein
  

  Mouse Semaphorin 4B (Leu31-Glu703) Accession # Q62179.2	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q62179.2
• N-terminal Sequence: Leu31
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 101-117 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

• KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B
• MGC131831
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B
• SEMA4B
• SEMAC
• Semaphorin 4B
• semaphorin-4B
• SemC

Background

Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, mouse Sema4B shares 85% and 95% aa identity with the human and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3-4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B^-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).

1. Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
2. Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
3. Schultze, W. et al. (2001) J. Neurochem. 78:482.
4. Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
5. Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
6. Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
7. Nagai, H. et al. (2007) Oncogene. 26:4025.

Bioinformatics

• Uniprot:
  • Q62179.2()