Recombinant Mouse P-Cadherin Fc Chimera Protein, CF

Images

 
There are currently no images for P-Cadherin (761-MP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse P-Cadherin Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 105 cells/well are added to Recombinant Mouse P‑Cadherin Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 40‑80% will adhere after 1.5 hours at 37 °C.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Mouse myeloma cell line, NS0-derived mouse P-Cadherin protein
Mouse P-Cadherin
(Glu100-Gly647)
Accession # Q8BSL6
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu100
Protein/Peptide Type
Recombinant Proteins
Gene
Cdh3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
87.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse P-Cadherin Fc Chimera Protein, CF

  • CAD3
  • cadherin 3, P-cadherin (placental)
  • cadherin 3, type 1, P-cadherin (placental)
  • Cadherin-3
  • CDH3
  • CDHP
  • CDHPcalcium-dependent adhesion protein, placental
  • HJMD
  • PCAD
  • PCadherin
  • P-Cadherin
  • PCADP-cadherin
  • Placental cadherin

Background

Placental Cadherin (P-Cadherin, PCAD, or Cadherin-3) is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region. Mutations in this gene have been associated with hypotrichosis with juvenile macular dystrophy (5). P-Cadherin over-expression is observed in various malignant tumors and is often correlated with increased tumor motility and invasiveness (6, 7).

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
  4. Nose, A. et al. (1987) EMBO J. 6:3655.
  5. J. Invest. Derm. (2002) 199:1210.
  6. Clin. Cancer Res. (2008) 14:6487.
  7. Oncogene (2010) 29:393.

Customers Who Viewed This Item Also Viewed...

H00001806-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
AF761
Species: Mu
Applications: WB, Simple Western, Flow, IHC, AdBlk, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP2-15840
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
H00005555-P01
Species: Hu
AF5415
Species: Hu
Applications: WB, IHC, ICC
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-16558
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-02710
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-22439
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-85896
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01437
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, IHC, IHC-P
AF1002
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-46290
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
761-MP
Species: Mu
Applications: Bioactivity

Publications for P-Cadherin (761-MP) (0)

There are no publications for P-Cadherin (761-MP).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for P-Cadherin (761-MP) (0)

There are no reviews for P-Cadherin (761-MP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for P-Cadherin (761-MP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106
Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse P-Cadherin Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Cdh3
Entrez
Uniprot