Recombinant Mouse Osteocrin Protein, CF

When Recombinant Mouse NPRC/NPR-3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6‑3.6 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Osteocrin Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
  
• Source: E. coli-derived mouse Osteocrin protein
  Val28-Gly130, with a C-terminal 6-His tag
• Accession #: P61364
• N-terminal Sequence: Val28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14-18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Osteocrin Protein, CF

• Musclin
• Osteocrin
• OSTN

Background

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acid C-terminal active peptide (1). It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles (2). Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osteocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

1. Nishizawa, H. et al. (2004) Journal of Biological Chemistry, 279:19391.
2. Thomas, G. et al. (2003) Journal of Biological Chemistry, 278:50563.
3. Jeramic N. et al. (2017) J Cell Physiol 232:61.
4. Subbatino, E. et al. (2015) Proc. Acad. Natl. Sci. U. S. A. 112:16042.
5. Kanai, Y. et al. (2017) J of Clin Invest 127: 4136
6. Ataman, B. et al. (2016) Nature 539:242.

Publications for Osteocrin (9700-ON)(1)

Publication using 9700-ON

• M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke Skeletal muscle derived Musclin protects the heart during pathological overload Nature Communications, 2022-01-10;13(1):149. 2022-01-10 [PMID: 35013221] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P61364()