Recombinant Mouse OSMR beta Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse OSMR beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse OSM R beta Fc Chimera is immobilized at 0.5 µg/mL and Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG) at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Mouse Oncostatin M/OSM (Catalog # 495-MO) that produces 50% of the optimal binding response is approximately 0.2‑1 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse OSMR beta protein
  

  Mouse OSM R beta (Glu24-Leu738) Accession # O70458	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: O70458
• N-terminal Sequence: Glu24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Osmr
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 109 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse OSMR beta Fc Chimera Protein, CF

• IL-31 receptor subunit beta
• IL-31R subunit beta
• IL-31RB
• IL-31R-beta
• Interleukin-31 receptor subunit beta
• MGC150627
• MGC75127
• oncostatin M receptor
• oncostatin-M specific receptor beta subunit
• oncostatin-M-specific receptor subunit beta
• OSM R beta
• OSMR beta
• OSMR
• OSMRB
• OSMRBMGC150626
• PLCA1

Background

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) residue type I transmembrane protein which contains a 23 aa residue signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity, but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta .

1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
2. Tanaka, M. et al. (1999) Blood 93:804.

Bioinformatics

• Gene Symbol: Osmr
• Uniprot:
  • O70458()