Recombinant Mouse LRRC32/GARP Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse LRRC32/GARP Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant mouse LRRC32/GARP is immobilized at 5 μg/mL, recombinant human Latent TGF-beta binds with an apparent K_d <25 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse LRRC32/GARP protein
  Ile18-Asn628, with a C-terminal 6-His tag
• Accession #: NP_001106850
• N-terminal Sequence: Ile18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Lrrc32
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 67.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, PEG 3350 and CHAPS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LRRC32/GARP Protein, CF

• D11S833E
• D11S833Eleucine-rich repeat-containing protein 32
• GARP
• GARPGarpin
• Garpin
• Glycoprotein A repetitions predominantgarpin
• leucine rich repeat containing 32
• LRRC32

Background

Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2-4). Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Among T cells, it is selectively expressed on activated FOXP3⁺ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6-8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).

1. Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296.
2. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213.
3. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307.
4. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545.
5. Macaulay, I.C. et al. (2007) Blood 109:3260.
6. Wang, R. et al. (2008) PloS ONE 3:e2705.
7. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439.
8. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343.
9. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445.
10. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315.
11. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.

Bioinformatics

• Gene Symbol: Lrrc32
• Uniprot:
  • NP_001106850()