Recombinant Mouse IL-3 Protein

Images

 
1 μg/lane of Recombinant Mouse IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 15 kDa.
Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 20-100 pg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Mouse IL-3 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Holmes, K.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The ED50 for this effect is 20-100 pg/mL.
Source
E. coli-derived mouse IL-3 protein
Asp33-Cys166, with an N-terminal Met
Accession #
N-terminal Sequence
Asp33
Protein/Peptide Type
Recombinant Proteins
Gene
Il3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
403-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-3 Protein

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

  1. Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
  2. Fung, M.C. et al., 1984, Nature 307:233.
  3. Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.

Customers Who Viewed This Item Also Viewed...

NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-13967
Species: Hu
Applications: IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
403-ML
Species: Mu
Applications: Bioactivity

Publications for IL-3 (403-ML)(95)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, In Vivo, In vivo injection.


Filter By Application
Bioassay
(92)
In Vivo
(2)
In vivo injection
(1)
All Applications
Filter By Species
Mouse
(95)
All Species
Showing Publications 1 - 10 of 95. Show All 95 Publications.
Publications using 403-ML Applications Species
R Berndt, L Hummitzsch, K He beta, M Albrecht, K Zitta, R Rusch, B Sarras, A Bayer, J Cremer, F Faendrich, J Gro beta Allogeneic transplantation of programmable cells of monocytic origin (PCMO) improves angiogenesis and tissue recovery in critical limb ischemia (CLI): a translational approach Stem Cell Res Ther, 2018;9(1):117. 2018 [PMID: 29703251] (Bioassay, Mouse) Bioassay Mouse
JP Robichaux, YY Elamin, Z Tan, BW Carter, S Zhang, S Liu, S Li, T Chen, A Poteete, A Estrada-Be, AT Le, A Truini, MB Nilsson, H Sun, E Roarty, SB Goldberg, JR Brahmer, M Altan, C Lu, V Papadimitr, K Politi, RC Doebele, KK Wong, JV Heymach Mechanisms and clinical activity of an EGFR and HER2 exon 20-selective kinase inhibitor in non-small cell lung cancer Nat. Med., 2018;0(0):. 2018 [PMID: 29686424] (Bioassay, Mouse) Bioassay Mouse
P Herbener, K Schönfeld, M König, M Germer, JM Przyborski, K Bernöster, J Schüttrump Functional relevance of in vivo half antibody exchange of an IgG4 therapeutic antibody-drug conjugate PLoS ONE, 2018;13(4):e0195823. 2018 [PMID: 29672587] (Bioassay, Mouse) Bioassay Mouse
HT Fang, CA El Farran, QR Xing, LF Zhang, H Li, B Lim, YH Loh Global H3.3 dynamic deposition defines its bimodal role in cell fate transition Nat Commun, 2018;9(1):1537. 2018 [PMID: 29670118] (Bioassay, Mouse) Bioassay Mouse
BA Gutierrez, MA Chavez, AI Rodarte, M Ramos, A Dominguez, Y Petrova, AJ Davalos, RM Costa, R Elizondo, MJ Tuvim, BF Dickey, AR Burns, R Heidelberg, R Adachi Munc18-2, but not Munc18-1 or Munc18-3, controls compound and single-vesicle regulated exocytosis in mast cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29599294] (Bioassay, Mouse) Bioassay Mouse
H Wang, L Bei, CA Shah, W Huang, LC Platanias, EA Eklund The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia Oncogene, 2018;0(0):. 2018 [PMID: 29459712] (Bioassay, Mouse) Bioassay Mouse
A Papaspyrop, L Bradley, A Thapa, CY Leung, K Toskas, D Koennig, DE Pefani, C Raso, C Grou, G Hamilton, N Vlahov, A Grawenda, S Haider, J Chauhan, L Buti, A Kanapin, X Lu, F Buffa, G Dianov, A von Kriegs, D Matallanas, A Samsonova, M Zernicka-G, E O'Neill RASSF1A uncouples Wnt from Hippo signalling and promotes YAP mediated differentiation via p73 Nat Commun, 2018;9(1):424. 2018 [PMID: 29382819] (Bioassay, Mouse) Bioassay Mouse
X Jiang, C Hu, K Ferchen, J Nie, X Cui, CH Chen, L Cheng, Z Zuo, W Seibel, C He, Y Tang, JR Skibbe, M Wunderlich, WC Reinhold, L Dong, C Shen, S Arnovitz, B Ulrich, J Lu, H Weng, R Su, H Huang, Y Wang, C Li, X Qin, J Mulloy, Y Zheng, J Diao, J Jin, C Li, PP Liu, C He, Y Chen, J Chen Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia Nat Commun, 2017;8(1):2099. 2017 [PMID: 29235481] (Bioassay, Mouse) Bioassay Mouse
N Okamoto, K Mizote, H Honda, A Saeki, Y Watanabe, T Yamaguchi-, R Fukui, N Tanimura, Y Motoi, S Akashi-Tak, T Kato, S Fujishita, T Kimura, U Ohto, T Shimizu, T Hirokawa, K Miyake, K Fukase, Y Fujimoto, Y Nagai, K Takatsu Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28754693] (Bioassay, Mouse) Bioassay Mouse
HJ Lee, MP Jedrychows, A Vinayagam, N Wu, N Shyh-Chang, Y Hu, C Min-Wen, JK Moore, JM Asara, CA Lyssiotis, N Perrimon, SP Gygi, LC Cantley, MW Kirschner Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation Cell Rep, 2017;20(3):721-736. 2017 [PMID: 28723573] (Bioassay, Mouse) Bioassay Mouse
Show All 95 Publications.

Reviews for IL-3 (403-ML) (0)

There are no reviews for IL-3 (403-ML). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for IL-3 (403-ML) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IL-3 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Il3
Entrez
Uniprot