Recombinant Mouse IL-20 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-20 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta . The ED 50 for this effect is 0.5-2.5 ng/mL.
• Source: E. coli-derived mouse IL-20 protein Leu25-Leu176, with an N-terminal Met
• Accession #: Q9JKV9.1
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il20
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 3 months, 2 to 8 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaH 2 PO 4 and NaCl.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-20 Protein, CF

• Cytokine Zcyto10
• IL10D
• IL20
• IL-20
• IL-20ZCYTO10four alpha helix cytokine
• interleukin 20
• interleukin-20
• MGC96907

Background

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL-20 R beta . The second complex is composed of IL-22 R and IL-20 R beta . Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1 - 6).

1. Blumberg, H. et al. (2001) Cell 104:9.
2. Liu, L. et al. (2003) Blood 102:3206.
3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.

Publications for IL-20 (1204-ML/CF)(4)

Publications using 1204-ML/CF

• WZ Ding, GY Han, HH Jin, CF Zhan, Y Ji, XL Huang Anti-IL-20 monoclonal antibody suppresses hepatocellular carcinoma progression Oncol Lett, 2018-09-05;16(5):6156-6162. 2018-09-05 [PMID: 30333881] (Bioassay, Mouse)
• Wang , Xiaoting, Ota , Naruhisa, Manzanillo , Paolo, Kates , Lance, Zavala-Solorio , Jose, Eidenschenk , Celine, Zhang , Juan, Lesch , Justin, Lee , Wyne P, Ross , Jed, Diehl , Lauri, van Bruggen , Nicholas, Kolumam , Ganesh, Ouyang , Wenjun Interleukin-22 alleviates metabolic disorders and restores mucosal immunity in diabetes. Nature, 2014-08-06;514(7521):237-41. 2014-08-06 [PMID: 25119041] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010-09-29;84(24):12703-12. 2010-09-29 [PMID: 20881038] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Il20
• Uniprot:
  • Q9JKV9.1()