Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

Images

 
2 μg/lane of Recombinant Mouse IGSF4B/SynCAM3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 35-44 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse IGSF4B/SynCAM3, immobilized at 1.25-2.5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Source
Mouse myeloma cell line, NS0-derived mouse IGSF4B/SynCAM3 protein
Asn23-His328, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn23
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
35-44 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF

  • BIgR
  • Brain immunoglobulin receptor
  • CADM3
  • cell adhesion molecule 3
  • IGSF4B
  • IGSF4BSynCAM3
  • Immunoglobulin superfamily member 4B
  • member 4B
  • Necl-1
  • NECL1TSLC1-like protein 1
  • Nectin-like protein 1
  • Synaptic cell adhesion molecule 3
  • SynCAM3
  • TSLC1-like 1
  • TSLL1
  • TSLL1SYNCAM3

Background

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl-1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48-50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 396 amino acid (aa) mouse IGSF4B contains a 22 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The IGSF4B ECD is highly conserved, sharing 95-96% aa identity between human, mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35-50% aa identity. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca2+‑independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl-2 (IGSF-4) in trans. It also contains two C2‑type Ig-like domains that are responsible for Ca2+-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). IGSF-4B was also identified as a tumor-suppressor gene and it inhibits migration, invasion, and induces differentiation of glioma cells (6). It also suppresses growth and tumorigenic ability of colon cancer cells (7). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons.
  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
  3. Fukami, T. et al. (2003) Gene 323:11.
  4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
  5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.
  6. Yin B. et al. (2009) Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 31:669.
  7. Raveh S. et al. (2009) J. Cell Biochem. 108:326.

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