Recombinant Mouse IFN-epsilon Protein

Recombinant Mouse IFN-epsilon (Catalog # 9147-ME) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 6.00-60.0 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IFN-epsilon Protein Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
• Source: E. coli-derived mouse IFN-epsilon protein
  Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
• Accession #: Q80ZF2
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-epsilon Protein

• IFNE
• IFN-E
• IFNE1PRO655
• IFNepsilon
• IFN-epsilon
• IFNT1
• Ifn-tau-11
• INFE1
• interferon epsilon 1
• interferon epsilon
• Interferon epsilon-1
• interferon tau-1
• interferon, epsilon
• interferon-epsilon
• MGC119018
• MGC119020

Background

Interferon-epsilon (IFN-epsilon ) is a secreted, approximately 18 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-epsilon shares 55% amino acid sequence identity with human and rat IFN-epsilon , respectively. IFN-epsilon signals through IFN-alpha / beta R1 and IFN-alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen
(2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8⁺ T cells to sites of virus infection (5, 7).

1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
6. Hermant, P. et al. (2013) PLoS One 8:e71320.
7. Day, S.L. et al. (2008) J. Immunol. 180:7158.

Bioinformatics

• Uniprot:
  • Q80ZF2()