Recombinant Mouse IFN-epsilon Protein, CF

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Recombinant Mouse IFN-epsilon (Catalog # 9147-ME/CF) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Mouse IFN-epsilon Protein, CF Summary

Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.2-1 ng/mL.
Source
E. coli-derived mouse IFN-epsilon protein
Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS
Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-epsilon Protein, CF

  • IFNE
  • IFN-E
  • IFNE1PRO655
  • IFNepsilon
  • IFN-epsilon
  • IFNT1
  • Ifn-tau-11
  • INFE1
  • interferon epsilon 1
  • interferon epsilon
  • Interferon epsilon-1
  • interferon tau-1
  • interferon, epsilon
  • interferon-epsilon
  • MGC119018
  • MGC119020

Background

Interferon-epsilon (IFN-epsilon ) is a secreted, approximately 18 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-epsilon shares 55% amino acid sequence identity with human and rat IFN-epsilon, respectively. IFN-epsilon signals through IFN-alpha / beta R1 and IFN-alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further upregulated in the uterus by estrogen (2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8+ T cells to sites of virus infection (5, 7).
  1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
  5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
  6. Hermant, P. et al. (2013) PLoS One 8:e71320.
  7. Day, S.L. et al. (2008) J. Immunol. 180:7158.

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