Recombinant Mouse IFN-epsilon Protein, CF

Recombinant Mouse IFN-epsilon (Catalog # 9147-ME/CF) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 6.00-60.0 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFN-epsilon Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
• Source: E. coli-derived mouse IFN-epsilon protein
  Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
• Accession #: Q80ZF2
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS
• Purity: >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-epsilon Protein, CF

• IFNE
• IFN-E
• IFNE1PRO655
• IFNepsilon
• IFN-epsilon
• IFNT1
• Ifn-tau-11
• INFE1
• interferon epsilon 1
• interferon epsilon
• Interferon epsilon-1
• interferon tau-1
• interferon, epsilon
• interferon-epsilon
• MGC119018
• MGC119020

Background

Interferon-epsilon (IFN-epsilon ) is a secreted, approximately 18 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-epsilon shares 55% amino acid sequence identity with human and rat IFN-epsilon , respectively. IFN-epsilon signals through IFN-alpha / beta R1 and IFN-alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further upregulated in the uterus by estrogen (2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8⁺ T cells to sites of virus infection (5, 7).

1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
6. Hermant, P. et al. (2013) PLoS One 8:e71320.
7. Day, S.L. et al. (2008) J. Immunol. 180:7158.

Bioinformatics

• Uniprot:
  • Q80ZF2()