Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse (rm) HVEM Fc Chimera is immobilized at 100 ng/ mL, 100 μL/well, the concentration of biotinylated rmBTLA Fc Chimera that produces 50% of the optimal binding response is found to be approximately 30-120 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse HVEM/TNFRSF14 protein
  

  Mouse HVEM (Gln39-Val207) Accession # NP_849262	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_849262
• N-terminal Sequence: Gln39
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf14
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 45.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HVEM/TNFRSF14 Fc Chimera Protein, CF

• ATAR
• CD270 antigen
• CD270
• CD40-like protein
• Herpes virus entry mediator A
• Herpesvirus entry mediator A
• HveA
• HVEM
• HVEMTR2HVEAATAR
• LIGHTR
• TNFRSF14
• tumor necrosis factor receptor superfamily member 14
• tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator)
• Tumor necrosis factor receptor-like 2
• tumor necrosis factor receptor-like gene2

Background

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature mouse HVEM consists of a 170 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 24 aa transmembrane segment, and a 45 aa cytoplasmic tail with a TRAF interaction domain (2). Within the ECD, mouse HVEM shares 55% and 89% aa sequence identity with human and rat HVEM, respectively. HVEM is highly expressed on naïve CD4⁺ T cells, CD8⁺ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (3-7). Its expression declines during effector T cell activation but is upregulated during Treg activation (3, 4). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (3, 8-11). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (7, 9) and contributes to Th1 inflammation and cardiac allograft rejection (12, 13). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3, 6, 8, 9) and dampens intestinal inflammation (14). HVEM enhances the development of CD8⁺ T cell memory and Treg function (4, 5). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (15). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (Montgomery, 8, 10, 16).

1. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
2. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
3. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
4. Tao, R. et al. (2008) J. Immunol. 180:6649.
5. Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
6. de Trez, C. et al. (2008) J. Immunol. 180:238.
7. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
8. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
9. Cai, G. et al. (2008) Nat. Immunol. 9:176.
10. Mauri, D.N. et al. (1998) Immunity 8:21.
11. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
12. Wang, J. et al. (2005) J. Immunol. 174:8173.
13. Ye, Q. et al. (2002) J. Exp. Med. 195:795.
14. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
15. Shui, J.W. et al. (2012) Nature 488:222.
16. Montgomery, R.I. et al. (1996) Cell 87:427.

Publications for HVEM/TNFRSF14 (2516-HV)(1)

Publication using 2516-HV

• H Kinosada, JI Yasunaga, K Shimura, P Miyazato, C Onishi, T Iyoda, K Inaba, M Matsuoka HTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors PLoS Pathog, 2017-01-03;13(1):e1006120. 2017-01-03 [PMID: 28046066] (Bead-based Bioassay)

Bioinformatics

• Gene Symbol: Tnfrsf14
• Uniprot:
  • NP_849262(Mouse)