Recombinant Mouse Glypican 1 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Glypican 1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmGlypican 1 at 3 µg/mL (100 µL/well) will bind rhFGF-basic with an apparent K_D < 1 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Glypican 1 protein
  Asp24-Ser529, with a C-terminal 6-His tag
• Accession #: Q9QZF2
• N-terminal Sequence: Asp24
• Protein/Peptide Type: Recombinant Proteins
• Gene: Gpc1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 56.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Glypican 1 Protein, CF

• FLJ38078
• Glypican 1
• glypican proteoglycan 1
• glypican
• glypican-1
• GPC1

Background

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that play a key role in growth factor signaling (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60 - 70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known. One subfamily contains Glypicans 1, 2, 4 and 6, while another subfamily contains Glypicans 3 and 5 (3, 5). Mouse Glypican 1 (GPC-1) is synthesized as a 557 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 506 aa mature segment, and a 28 aa C-terminal prosegment (6, 7). There are two potential N-linked, and four potential O-linked sites for glycosylation or glycanation. There is at least one heparan sulfate (HS) modification on GPC-1 that contributes to a native molecular weight of approximately 110 kDa (8, 9). Mature mouse GPC-1 shares 91% and 98% aa identity with mature human and rat GPC-1, respectively. There are two potential splice variants of mouse GPC-1. The first is truncated and shows a seven amino acid substitution for the first 294 aa; the second reveals an alternate start site at Met73 (10, 11). Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells (8, 9, 12 - 15). The function of GPC-1 is complex and varied. As a proteoglycan, it appears to make use of its HS adduct to impact select growth factor activity (16). GPC-1 accomplishes its co-receptor role by having juxtramembrane HS attachment sites and a flexible, GPI-linkage (17). Data suggests GPC-1 and sulfation enzymes may collaborate to regulate FGF signaling. HS modules that are rich in 2-O- and 6-O- sulfate upregulate FGF-2 activation of FGFR1c (18). Similarly, FGF-1 requires both 2-O- and 6-O-sulfation to bind to FGFR2c and 3c. By contrast, FGF-1 requires no sulfation to bind to FGFR2b, and FGF-8b needs only 6-O-sulfation to activate FGFR3c. Thus, many FGF receptor isoform specific effects may be attributed to an interaction between Glypican family members and the cell sulfation system (19).

1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
2. Fransson, L-A. et al. (2004) Cell. Mol. Life Sci. 61:1016.
3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
4. Lamoureux, F. et al. (2007) BioEssays 29:758. 
5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968. 
6. GenBank Accession # Q9QZF2. 
7. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
8. Litwick, E.D. et al. (1994) J. Neurosci. 14:3713.
9. Litwick, E.D. et al. (1998) Dev. Dyn. 211:72. 
10. GenBank Accession # EDL39991.
11. GenBank Accession # EDL39993.
12. Chernousov, M.A. et al. (2006) J. Neurosci. 26:508.
13. Wegrowski, Y. et al. (2006) Clin. Exp. Immunol. 144:485.
14. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
15. Kayed, H. et al. (2006) Int. J. Oncol. 29:1139.
16. Selleck, S.B. (2006) SciSTKE April 4:pe17.
17. Qiao, D. et al. (2003) J. Biol. Chem. 278:16045.
18. Su, G. et al. (2006) Am. J. Pathol. 168:2014.
19. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.

Publications for Glypican 1 (4520-GP)(1)

Publication using 4520-GP

• Nakajima, C;Sawada, M;Umeda, E;Takagi, Y;Nakashima, N;Kuboyama, K;Kaneko, N;Yamamoto, S;Nakamura, H;Shimada, N;Nakamura, K;Matsuno, K;Uesugi, S;Vep?ek, NA;Küllmer, F;Nasufovi?, V;Uchiyama, H;Nakada, M;Otsuka, Y;Ito, Y;Herranz-Pérez, V;García-Verdugo, JM;Ohno, N;Arndt, HD;Trauner, D;Tabata, Y;Igarashi, M;Sawamoto, K; Identification of the growth cone as a probe and driver of neuronal migration in the injured brain Nature communications 2024-03-09 [PMID: 38461182] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Gpc1
• Uniprot:
  • Q9QZF2()