Recombinant Mouse Fibulin 7 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Fibulin 7 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED₅₀ for this effect is 0.7-3.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Fibulin 7 protein
  Gln25-Phe440, with an N-terminal HA Tag
• Accession #: Q501P1
• N-terminal Sequence: Tyr
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Fbln7
• Purity: >90%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 53-63 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Fibulin 7 Protein, CF

• FBLN7
• FIBL-7
• Fibulin 7
• TM14

Background

Fibulin 7, also known as TM14, is an approximately 46 kDa member of the fibulin family of proteins (1, 2). Structurally, Fibulin 7 contains an N-terminal Sushi domain, 3 central tandem EGF modules, and a C-terminal domain homologous to the fibulin module (2). Mouse Fibulin 7 mRNA is highly expressed in teeth, with weaker expression in brain, kidneys, muscles, and bones (2). Mouse Fibulin 7 shares 86% amino acid sequence identity with human Fibulin 7. Fibulin 7 interacts with several extracellular molecules, including Fibronectin, DSPP, and Fibulin 1 (2). A C-terminal fragment of Fibulin 7 was able to bind Human Umbilical Vein Endothelial Cells (HUVECs) and inhibit HUVEC tube formation and vessel sprouting in aortic ring assays, suggesting that Fibulin 7 may have anti-angiogenic activity (3). Fibulin 7 may be important for proper development, since it is included in a chromosomal deletion that is associated with severe cardiac defects and craniofacial anomalies (4).

1. Gallagher, W.M. et al. (2005) Trends Mol. Med. 11:336.
2. de Vega, S. et al. (2007) J. Biol. Chem. 282:30878.
3. de Vega, S. et al. (2014) Arch. Biochem. Biophys. 545:148.
4. Russell, M.W. et al. (2014) Hum. Mol. Genet. 23:4272.

Bioinformatics

• Gene Symbol: Fbln7
• Uniprot:
  • Q501P1()