Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphA2 Fc Chimera (Catalog # 639-A2) at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse Ephrin‑A1 Fc Chimera with a linear range of 0.078‑5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
Mouse Ephrin-A1
(Asp19-Ser182)
Accession # P52793
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efna1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Applications/Dilutions

Theoretical MW
46.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
BT602 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions

Reconstitute at 100 μg/mL with sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein

  • B61
  • ECKLG
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • LERK1LERK-1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4

Background

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21‑25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10‑12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
  4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
  5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
  6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
  7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
  8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  9. Ogawa, K. et al. (2000) Oncogene 19:6043.
  10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
  11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
  12. Pandey, A. et al. (1995) Science 268:567.
  13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

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Publications for Ephrin-A1 (BT602)(3)

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Other Available Formats

Biotin BT602

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Bioinformatics

Gene Symbol Efna1
Uniprot