Recombinant Mouse CRIM1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CRIM1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Glienke, J. et al. (2002) Mech. Dev. 119:165.

  rmCRIM1 immobilized at 3 μg/mL, 100 μL/well, will mediate >50% NIH3T3 cell adhesion (added at 1 x 10⁵ cells/well).

• Source: Mouse myeloma cell line, NS0-derived mouse CRIM1 protein
  Leu35-Ser940, with a C-terminal 6-His tag
• Accession #: Q9JLL0
• N-terminal Sequence: Leu35
• Structure / Form: Noncovalently-linked oligomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Crim1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 100.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRIM1 Protein, CF

• CRIM1
• CRIM-1
• cysteine rich transmembrane BMP regulator 1 (chordin-like)
• cysteine-rich motor neuron 1 protein
• cysteine-rich motor neuron 1
• Cysteine-rich repeat-containing protein S52
• MGC138194
• S52

Background

CRIM1 (cysteine-rich motor neuron 1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors (1 - 4). The ~130 kDa, 1037 amino acid (aa) CRIM1 contains a 34 aa signal sequence, a 906 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 76 aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell, presumably by proteolytic processing (4). Mouse CRIM1 ECD shares 88%, 97%, 94%, 93% and 89% aa identity with human, rat, canine, equine and bovine CRIM1 ECD, respectively. CRIM1 CRR domains can interact with several cystine knot-containing growth factors, including VEGF-A, PDGF A and B, and BMPs 2, 4 and 7 (4, 5). It appears to bind VEGF or BMPs intracellularly, and antagonize them by lowering their expression, processing and secretion (4, 5). CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons (1, 6). It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation (2). Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing (2). CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis (6, 7). In adult kidney, it is expressed in glomeruli and the peritubular vasculature, where VEGF-A is constitutively expressed (5, 8). It is thought to regulate delivery of VEGF-A by podocytes to the kidney endothelium (5).

1. Kolle, G. et al. (2000), Mech. Dev. 90:181.
2. Glienke, J. et al. (2002) Mech. Dev. 119:165.
3. Abreu, J. G. et al. (2002) Gene 287:39.
4. Wilkinson, L. et al. (2003) J. Biol. Chem. 278:34181.
5. Wilkinson, L. et al. (2007) J. Am. Soc. Nephrol. 18:1697.
6. Kolle, G. et al. (2003) Dev. Dyn. 226:107.
7. Pennisi, D. J. et al. (2007) Dev. Dyn. 236:502.
8. Wilkinson, L. et al. (2009) Kidney Int. 76:1161.

Bioinformatics

• Gene Symbol: Crim1
• Uniprot:
  • Q9JLL0()