Recombinant Mouse CRELD1 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CRELD1 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.3-1.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CRELD1 protein
  Gln30-Glu362, with a C-terminal 6-His tag
• Accession #: Q91XD7
• N-terminal Sequence: No results obtained: Gln30 predicted, sequencing might be blocked
• Protein/Peptide Type: Recombinant Proteins
• Gene: Creld1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRELD1 Protein, CF

• atrioventricular septal defect 2
• AVSD2
• CIRRIN
• CRELD1
• cysteine-rich with EGF-like domain protein 1
• cysteine-rich with EGF-like domains 1
• DKFZp566D213

Background

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa (1). Within the large ECD, mouse and human CRELD1 share 92% aa sequence identity. CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro-apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha‑1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). R&D Systems in-house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.

1. Rupp, P.A. et al. (2002) Gene 293:47.
2. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225.
3. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169.
4. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.

Bioinformatics

• Gene Symbol: Creld1
• Uniprot:
  • Q91XD7()