Recombinant Mouse CRACC/SLAMF7 Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse CRACC/SLAMF7 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Recombinant Mouse CRACC/SLAMF7. Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CRACC/SLAMF7 protein
  Ala22-Gly224, with a C-terminal 10-His tag
• Accession #: Q8BHK6
• N-terminal Sequence: Ala22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Slamf7
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 23.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRACC/SLAMF7 Protein, CF

• 19A
• CD2 subset 1
• CD2-like receptor activating cytotoxic cells
• CD319 antigen
• CD319
• CRACC
• CRACCCD2-like receptor-activating cytotoxic cells
• CS119A24 protein
• Membrane protein FOAP-12
• novel LY9 (lymphocyte antigen 9) like protein
• Novel Ly9
• Protein 19A
• SLAM family member 7
• SLAMF7

Background

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19% - 35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8⁺ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4 - 7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).

1. Veillette, A. (2006) Immunol. Rev. 214:22.
2. Tovar, V. et al. (2002) Immunogenetics 54:394.
3. Murphy, J.J. et al. (2002) Biochem. J. 361:431.
4. Bouchon, A. et al. (2001) J. Immunol. 167:5517.
5. Lee, J.K. et al. (2007) J. Immunol. 179:4672.
6. Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
7. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
8. Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Bioinformatics

• Gene Symbol: Slamf7
• Uniprot:
  • Q8BHK6()