Recombinant Mouse CLEC9a Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CLEC9a Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by flow cytometry for its ability to bind a ligand expressed in necrotic EL‑4 mouse lymphoblast cells. When 100 ng of Recombinant Mouse CLEC9a Fc Chimera is added to 5 x 10⁵ EL-4 cells, >50% of necrotic EL-4 cells bind to the protein.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CLEC9a protein
  

  MDP	Mouse IgG2A (Glu98-Lys330)	IEGR	Mouse CLEC9a (Lys57-Ile264) Accession # NP_001192292
  N-terminus			C-terminus

• Accession #: NP_001192292
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Clec9a
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CLEC9a Fc Chimera Protein, CF

• CD370
• CLEC9a
• C-type lectin domain family 9 member A
• C-type lectin domain family 9, member A
• DNGR1
• HEEE9341
• UNQ9341

Background

CLEC9A (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C‑type lectin superfamily (1). Mature mouse CLEC9A consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM-like motif, a 21 aa transmembrane segment, and a 182 extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD) (2‑4). Within the ECD, mouse CLEC9A shares 57% and 80% aa sequence identity with human and rat CLEC9A, respectively. Alternative splicing of mouse CLEC9A generates additional isoforms with insertions in the stalk region or deletions of the transmembrane segment, the CTLD, or a portion of the CTLD (2). Although the CTLD of CLEC9A structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9A is expressed as a disulfide‑linked homodimer of approximately 50 kDa N‑glycosylated subunits (2, 4). Human CLEC9A expression is restricted to a subpopulation of BDCA‑3⁺ conventional dendritic cells (cDC) and CD16^- monocytes (2‑5). BDCA-3⁺ cDC are analagous to mouse CD8⁺ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules (6). In mouse, CLEC9A is additionally expressed on plasmacytoid dendritic cells (3, 4). CLEC9A ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming (2‑5, 7). Its recognition of filamentous actin in dead cells is important for triggering an immune response to necrotic cell debris (8-10). CLEC9A is also required for the presentation of viral proteins and the subsequent CTL‑mediated killing of virus-infected cells (11, 12). CLEC9A signaling triggers activation of the tyrosine kinase Syk (2, 8).

1. Kerrigan, A.M. and G.D. Brown (2010) Immunol. Rev. 234:335.
2. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693.
3. Caminschi, I. et al. (2008) Blood 112:3264.
4. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098.
5. Schreibelt, G. et al. (2012) Blood 119:2284.
6. Dudziak, D. et al. (2007) Science 315:107.
7. Poulin, L.F. et al. (2010) J. Exp. Med. 207:1261.
8. Sancho, D. et al. (2009) Nature 458:899.
9. Ahrens, S. et al. (2012) Immunity 36:635.
10. Zhang, J.-G. et al. (2012) Immunity 36:646.
11. Iborra, S. et al. (2012) J. Clin. Invest. 122:1628.
12. Zelenay, S. et al. (2012) J. Clin. Invest. 122:1615.

Review for CLEC9a (6776-CL) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer ELISA 03/30/2025

Summary

• Application: ELISA
• Lot: TNM0424061

Bioinformatics

• Gene Symbol: Clec9a
• Uniprot:
  • NP_001192292()