Recombinant Mouse Chordin-Like 2 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Chordin-Like 2 Protein Summary

• Details of Functionality: Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.2-1 µg/mL in the presence of 30 ng/mL of rhBMP-4.
• Source: Mouse myeloma cell line, NS0-derived mouse Chordin-like 2/CHRDL2 protein
  Gln24-Leu426, with a C-terminal 6-His tag
• Accession #: Q8VEA6.1
• N-terminal Sequence: No results obtained: Gln24 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: Chrdl2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Chordin-Like 2 Protein

• BNF-1
• Chordin like 2
• Chordin-like 2
• chordin-like protein 2
• CHRDL2

Background

Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is an approximately 55 kDa secreted glycoprotein that acts as a BMP antagonist. Mature mouse CHL2 contains three von Willebrand type C repeats and shares 73% and 95% amino acid sequence identity with human and rat CHL2, respectively (1-3). Alternative splicing of mouse Chordin-like 2 generates additional isoforms with substituted N-terminal or C-terminal regions (3). CHL2 is expressed in chondrocytes in developing joint cartilage (2, 4), osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder) (1-3). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG (2, 3, 5, 6). It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses (2, 5). CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation (2).

1. Wu, I. and M.A. Moses (2003) Gene 311:105.
2. Nakayama, N. et al. (2004) Development 131:229.
3. Oren, A. et al. (2004) Gene 331:17.
4. Lorda-Diez, C.I. et al. (2013) PLoS One 8:e60423.
5. Fujisawa, T. et al. (2009) Biochem. Biophys. Res. Commun. 385:215.
6. Zhang, J.L. et al. (2007) J. Biol. Chem. 282:20002.

Publications for Chordin-like 2/CHRDL2 (2520-CH)(1)

Publication using 2520-CH

• Alison C. McGarvey, Stanislav Rybtsov, Céline Souilhol, Sara Tamagno, Ritva Rice, David Hills et al. A molecular roadmap of the AGM region reveals BMPER as a novel regulator of HSC maturation Journal of Experimental Medicine 2017-12-04 [PMID: 29093060] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Chrdl2
• Uniprot:
  • Q8VEA6.1()