Recombinant Mouse CDNF Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CDNF Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 6-25 μg/mL on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived mouse CDNF protein
  Leu27-Leu187, with a C-terminal 10-His tag
• Accession #: Q8CC36
• N-terminal Sequence: Leu27
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cdnf
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CDNF Protein, CF

• arginine-rich, mutated in early stage tumors-like 1
• ARMETL1
• ARMET-like protein 1
• CDNF
• cerebral dopamine neurotrophic factor
• Conserved dopamine neurotrophic factor

Background

CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1), is a 17 - 19 kDa secreted protein that shares 52% amino acid (aa) identity with mouse MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet (1). The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1 - 3). The mouse CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).

 

1. Lindholm, P. et al. (2007) Nature 448:73.
2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.

Publications for CDNF (5187-CD)(2)

Publications using 5187-CD

• A Eesmaa, LY Yu, H Göös, T Danilova, K Nõges, E Pakarinen, M Varjosalo, M Lindahl, P Lindholm, M Saarma CDNF Interacts with ER Chaperones and Requires UPR Sensors to Promote Neuronal Survival International Journal of Molecular Sciences, 2022-08-22;23(16):. 2022-08-22 [PMID: 36012764] (ELISA Standard, N/A)
• E Galli, P Lindholm, LS Kontturi, M Saarma, A Urtti, M Yliperttul Characterization of CDNF-Secreting ARPE-19 Cell Clones for Encapsulated Cell Therapy Cell Transplant, 2019-03-06;28(4):413-424. 2019-03-06 [PMID: 30841717] (ELISA Control, Human)

Bioinformatics

• Gene Symbol: Cdnf
• Uniprot:
  • Q8CC36(Mouse)