Recombinant Mouse CD6 His Tagged Protein, CF

Images

 
2 μg/lane of Recombinant Mouse CD6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 70 - 80 kDa.
Immobilized Recombinant Mouse CD6 (Catalog # 9946-CD)supports the adhesion of HuT 78 human cutaneous T cell lymphoma cells. TheED50 for this effect is 0.4-2.4 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse CD6 His Tagged Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD6 protein
Leu18-Gly396, with a C-Terminal 6-His tag
Accession #
N-terminal Sequence
Leu18
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-80 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD6 His Tagged Protein, CF

  • CD6 antigenFLJ44171
  • CD6 molecule
  • CD6
  • T12
  • Tp120
  • TP120T-cell differentiation antigen CD6

Background

CD6 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, which is characterized by the presence of one or several repeats of SRCR domains in their extracellular region (1). CD6 is a type I transmembrane glycoprotein and contains three extracellular SRCR domains (2, 3). It is expressed on thymocytes, T cells, a subset of B cells, and on certain regions of the brain (3, 4). Mouse CD6 is a 665 amino acid (aa) protein that includes a 16 aa signal sequence, a 382 aa extracellular domain, a 21 aa transmembrane segment, and a 246 aa cytoplasmic region (5, 6). Within the ECD, mouse CD6 shares 69% and 88% aa sequence identity with human and rat CD6, respectively. CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6-specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule (7-12). Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6. It has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development (9, 13). Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction (9, 13). Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb (8, 10, 11, 14). The CD6 intracellular domain contains regions that can interact with SH2 or SH3-containing proteins. However, the signaling pathways have not been elucidated (5, 15, 16).
  1. Sarrias, M. et al. (2007) Proc. Natl. Acad. Sci. USA. 104:11724.
  2. Chappell, P. et al. (2015) Structure. 23:1426.
  3. Whitney, G.S. et al. (1995) J. Biol. Chem. 270:18187.
  4. Mayer, B. et al. (1990) J. Neuroimmunol. 29:193.
  5. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  6. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  7. Gangemi, R. et al. (1989) J. Immunol. 143:2439.
  8. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  9. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  10. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  11. Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  14. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  15. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  16. Whitney, G. et al. (1995) Mol. Immunol.  32:89.

Customers Who Viewed This Item Also Viewed...

NBP2-61727
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, MiAr
NB100-64852
Species: Hu
Applications: Flow, IHC, IHC-Fr
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP1-83192
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-80104
Species: Hu
Applications: WB
H00029956-M01A
Species: Hu, Mu
Applications: WB, ELISA, IHC
NBP2-20214
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57642
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-88582
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-25250
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF1172
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF1636
Species: Hu
Applications: WB, Simple Western, Flow, AgAct, CyTOF-ready, ICC
NB200-585
Species: Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
H00058516-B02P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
H00006124-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
AF3565
Species: Mu
Applications: WB, IHC, IP
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc

Publications for CD6 (9946-CD) (0)

There are no publications for CD6 (9946-CD).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CD6 (9946-CD) (0)

There are no reviews for CD6 (9946-CD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CD6 (9946-CD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse CD6 His Tagged Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot