Recombinant Mouse CD23/Fc epsilon RIIA Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse CD23/Fc epsilon RIIA Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When recombinant mouse CD23 is immobilized at 5 μg/mL (100 μL/well), it binds to mouse IgE in a range of 0.02-10 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD23/Fc epsilon RII protein
Glu50-Pro331, with an N-terminal 6-His tag
Accession #
N-terminal Sequence
His
Protein/Peptide Type
Recombinant Proteins
Gene
Fcer2a
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-43 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD23/Fc epsilon RIIA Protein, CF

  • BLAST-2
  • CD23
  • CD23A
  • CD23CD23 antigen
  • CLEC4J
  • CLEC4JC-type lectin domain family 4 member J
  • C-type lectin domain family 4, member J
  • Fc epsilon RII
  • Fc fragment of IgE, low affinity II, receptor for (CD23)
  • FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A)
  • fc-epsilon-RII
  • FCER2
  • Fcer2a
  • FceRII
  • IGEBF
  • Immunoglobulin E-binding factor
  • low affinity immunoglobulin epsilon Fc receptor
  • Ly-42
  • Lymphocyte IgE receptor

Background

The low affinity receptor for IgE, Fc epsilon RII (designated CD23) is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1-4). Mouse CD23 is a
45-49 kDa type II transmembrane glycoprotein that binds mouse, but not human, IgE (1-4). The longest isoform is 331 amino acids (aa) in length and contains a 23 aa cytoplasmic domain, a 26 aa transmembrane segment, and a 282 aa extracellular domain (ECD). The ECD C-type lectin domain binds both protein and carbohydrate, with separate sites for binding IgE, CD21, and beta 2 and alpha v integrins (2).  Coiled-coil topography within a connecting stalk contributes to oligomerization, which increases IgE affinity (1, 2). Mouse CD23 shares 88% aa identity with rat CD23 and up to 57% aa identity with isoforms of human, equine, porcine and bovine CD23. Isoforms which vary in the cytoplasmic sequence include the “a” isoform, which begins with the sequence MEENEYS and is constitutively expressed by B cells, and the “b” isoform, which begins with MDTHHT and is induced by IL-4 on a variety of inflammatory cells, B cells and epithelia (2, 5-7). The isoforms differentially participate in IgE-mediated endocytosis and phagocytosis (2, 8-10). CD23b and two 309 aa mouse CD23b isoforms, lacking either exon 5 or 6, also display distinct endocytic properties on intestinal epithelia (6, 11). Several soluble forms of CD23 are mainly generated by metalloprotease (especially ADAM10) and cysteine protease digestion, although potentially soluble mouse isoforms have also been sequenced (12-14). Both soluble and membrane-bound forms of CD23 include the lectin domain and show bioactivity. CD23 binding to monocyte integrins results in oxidative product generation and proinflammatory cytokine release (15). On human, but not mouse, B cells, sCD23 induces IgE secretion by binding CD21 (2, 9). In both, secreted IgE will bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (2).

  1. Kijimoto-Ochiai, S. (2002) Cell. Mol. Life Sci. 59:648.
  2. Gould, H. and B.J. Sutton (2008) Nat. Rev. Immunol. 8:205.
  3. Bettler, B. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7566.
  4. Gollnick, S.O. et al. (1990) J. Immunol. 144:1974.
  5. Kondo, H. et al. (1994) Int. Arch. Allergy Immunol. 105:38.
  6. Yu, L.C.H. et al. (2003) Am. J. Physiol. Gastrointest. Liver Physiol. 285:G223.
  7. Sukumar, S. et al. (2006) J. Immunol. 176:4811.
  8. Karagiannis, S.N. et al. (2001) Immunology 103:319.
  9. Aubry, J-P. et al. (1992) Nature 358:505.
  10. Sarfati, M. and G. Delespeese (1988) J. Immunol. 141:2195.
  11. Montagnac, G. et al. (2005) Traffic 6:230.
  12. Weskamp, G. et al. (2006) Nat. Immunol. 7:1293.
  13. Schulz, O. et al. (1997) Eur. J. Immunol. 27:584.
  14. Entrez Accession # EDL21949, EDL21951.
  15. Lecoanet-Henchoz, S. et al. (1995) Immunity 3:119.

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Bioinformatics

Gene Symbol Fcer2a
Uniprot
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