Recombinant Mouse Caspr2 His-tag Protein, CF

2 μg/lane of Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 130-150 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Caspr2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Caspr2 His-tag (Catalog # 10196-CR) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Human Contactin-1 Fc Chimera (Catalog # 904-CN) that produces 50% of the optimal binding response is 5-30 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Caspr2 protein
  Ala28-Ser1262, with a C-terminal 6-His tag
• Accession #: Q9CPW0
• N-terminal Sequence: Ala28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 138 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Caspr2 His-tag Protein, CF

• AUTS15
• Caspr2
• CASPR2DKFZp781D1846
• CDFE
• Cell recognition molecule Caspr2
• CNTNAP2
• contactin associated protein-like 2
• contactin-associated protein 2
• contactin-associated protein-like 2
• homolog of Drosophila neurexin IV
• KIAA0868
• NRXN4
• NRXN4Caspr2
• PTHSL1

Background

Contactin Associated Protein-Like 2 (CASPR2), also known as Cntnap2, is a neuronal type I transmembrane member of the neurexin family of adhesion molecules (ref). CASPR2, along with CASPR1 (or Cntnap1), is required for radical and longitudinal organization of myelinated axons (1). Mature mouse CASPR2 consists of a large extracellular domain (ECD) containing four Laminin G-like domains as well as two EFG-like domains and a Fibrinogen C-terminal domain, a transmembrane segment, and a short cytoplasmic domain. The ECD of mouse CASPR2 shares 94% and 93% amino acid (aa) identity with the human and rat ECD, respectively. CASPR2 is thought to be important for the control of neuron-glia interactions and is associated with alterations in synaptic structure, neuronal network activity, and an autism-related phenotype in mice (2, 3). In myelinated nerves, CASPR2 is confined to the juxtaparanodal region of the axon where it appears to associate with the immunoglobulin domains of TAG-1 (transient axonal glycoprotein 1) to form a scaffold, which clusters the potassium channels Kv1.1 and Kv1.2 (4). Recent studies demonstrate that CASPR2 binds to contactin1 (5).

1. Gordon A. et al. (2014) J. Neurosci. 10:1523.
2. Cope, E. et al. (2016) eNeuro. 0196-16.
3. Peñagarikano O. et al. (2011) Cell. 147(1):236.
4. Poliak, S. et al. (1999) Neuron 24:1037.
5. Rubio-Marrero, E. N. et al. (2016) J. Bio. Chem. 291:5788.

Bioinformatics

• Uniprot:
  • Q9CPW0()