>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60 kDa, reducing conditions
Read Publication using 5658-CE in the following applications:
Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
Dilute rmCEL to 0.2 ng/µL in Assay Buffer.
Dilute Substrate to 2 mM in Assay Buffer.
Load into the wells of a clear microplate 50 μL of 0.2 ng/µL rmCEL, and start the reaction by adding 50 µL of Substrate. Include a Blank Control containing 50 µL of Assay Buffer and 50 µL of Substrate without any rmCEL.
Incubate 10 minutes at room temperature.
Quickly add 100 µL of Reading Buffer to all wells (this will not stop the reaction).
Read immediately at a wavelength of 410 nm (bottom read) in endpoint mode.
Calculate specific activity:
Specific Activity (pmol/min/µg) =
Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)
*Adjusted for Substrate Blank
**Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).
rmCEL: 0.010 µg
Substrate: 0.5 mM
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF
Carboxyl ester lipase, also known as bile salt-stimulated lipase, belongs to the Type-B carboxyl esterase/lipase family of enzymes. The enzyme possesses a Ser‑His‑Asp catalytic triad, and is capable of hydrolyzing both ester and amide bonds. CEL is a non-specific lipase which hydrolyzes a variety of substrates, including cholesteryl esters, acylglycerols, and ceramide (1). CEL is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract, where it contributes to the digestion of dietary lipids (2). It is expressed at much lower levels by the liver, macrophages, and endothelial cells (3). CEL is present in the circulation, where it may play a role in low density lipoprotein metabolism and atherogenesis (4).
Wang, C.S. and J.A. Hartsuck, J.A. (1993) Biochim. Biophys. Acta. 1166:1.
Lombardo, D. (2001) Biochim. Biophys. Acta. 1533:1.
Hui, D.Y. and Howles, P.N. (2002) J. Lipid Res. 43:2017.
Brodt-Eppley, J. et al. (1995) Biochim. Biophys. Acta. 1272:69.
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