Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications EnzAct
Format
Carrier-Free

Order Details

Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF Summary

Details of Functionality
Measured by its ability to cleave p-Nitrophenyl butyrate (PNPB). The specific activity is >4,000 pmol/min/μg, as measured under the described conditions. 
Source
Mouse myeloma cell line, NS0-derived
Ala21-Leu534, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala21
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Enzymes
Gene
Cel
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publication using
5658-CE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Buffer: 25 mM Tris, pH 7.0
  • Reading Buffer: 25 mM Tris, pH 8.0
  • Recombinant Mouse Carboxyl Ester Lipase/CEL (rmCEL) (Catalog # 5658-CE)
  • Substrate: p-Nitrophenyl butyrate (PNPB) (Sigma, Catalog # N9876), 100 mM stock in acetone
  • 96-well Clear Plate (Catalog # DY990)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmCEL to 0.2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 2 mM in Assay Buffer.
  3. Load into the wells of a clear microplate 50 μL of 0.2 ng/µL rmCEL, and start the reaction by adding 50 µL of Substrate. Include a Blank Control containing 50 µL of Assay Buffer and 50 µL of Substrate without any rmCEL.
  4. Incubate 10 minutes at room temperature.
  5. Quickly add 100 µL of Reading Buffer to all wells (this will not stop the reaction).
  6. Read immediately at a wavelength of 410 nm (bottom read) in endpoint mode.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).

Per Well:
  • rmCEL: 0.010 µg
  • Substrate: 0.5 mM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Carboxyl Ester Lipase/CEL Protein, CF

  • BAL
  • bile salt-activated lipase
  • Bile salt-stimulated lipase
  • BSDL
  • BSSL
  • BSSLbile salt-dependent lipase, oncofetal isoform
  • bucelipase
  • carboxyl ester hydrolase
  • carboxyl ester lipase (bile salt-stimulated lipase)
  • Carboxyl Ester Lipase
  • CEase
  • CEL
  • CELL
  • Cholesterol esterase
  • EC 3.1.1
  • EC 3.1.1.13
  • EC 3.1.1.3
  • FAP
  • FAPP
  • fetoacinar pancreatic protein
  • LIPA
  • lysophospholipase, pancreatic
  • MODY8bile-salt-activated lipase
  • Pancreatic lysophospholipase
  • Sterol esterase

Background

Carboxyl ester lipase, also known as bile salt-stimulated lipase, belongs to the Type-B carboxyl esterase/lipase family of enzymes. The enzyme possesses a Ser‑His‑Asp catalytic triad, and is capable of hydrolyzing both ester and amide bonds. CEL is a non-specific lipase which hydrolyzes a variety of substrates, including cholesteryl esters, acylglycerols, and ceramide (1). CEL is highly expressed by pancreatic acinar cells and secreted into the gastrointestinal tract, where it contributes to the digestion of dietary lipids (2). It is expressed at much lower levels by the liver, macrophages, and endothelial cells (3). CEL is present in the circulation, where it may play a role in low density lipoprotein metabolism and atherogenesis (4).

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Bioinformatics

Gene Symbol Cel
Entrez
Uniprot