Recombinant Mouse Cadherin-6/KCAD Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Cadherin-6/KCAD Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of Caki‑2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 0.2-1.0 μg/mL after a 30 minutes incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse Cadherin-6/KCAD protein
Met1-Ala615 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn22 (pro-protein) & Ser54 (mature protein)
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
67.0 kDa (pro-protein) & 63.3 kDa (mature protein).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-105 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in DPBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Cadherin-6/KCAD Protein, CF

  • cadherin 6, type 2, K-cadherin (fetal kidney)
  • cadherin, fetal kidney
  • Cadherin6
  • Cadherin-6
  • CDH6
  • KCAD
  • K-cadherin (fetal kidney)
  • K-Cadherin

Background

Cadherin‑6, also known as KCAD or K‑Cadherin, is a 110‑120 kDa type I transmembrane glycoprotein belonging to the classical Cadherin superfamily of
calcium‑dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Mouse Cadherin‑6 is synthesized with a 18 amino acid (aa) signal peptide and a 35 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 562 amino acid (aa) extracellular domain (ECD) that contains five tandem Cadherin repeats, a 21 aa transmembrane segment, and a 154 aa cytoplasmic domain (3). Within the ECD, mouse Cadherin‑6 shares 96% aa sequence identity with human and rat Cadherin‑10. It interacts homotypically as well as heterotypically with Cadherin‑9 and more weakly with Cadherins‑7, ‑10, and ‑14 (4, 5). Cadherin‑6 is expressed primarily in the kidney and brain, both during development and in the adult (6‑9). It is enriched in renal proximal tubule epithelial cells and their precursors at sites of cell‑cell contact as well as on basolateral membranes (7, 10, 11). In the nervous system, Cadherin‑6 is neuronally expressed in discrete regions of the neural plate, neural crest cells that give rise to peripheral nerves, and the brain (3, 6). Cadherin‑6 is additionally expressed in the developing retina, osteoclasts and their progenitors, ovarian epithelium, and endometrium during the follicular phase of the menstrual cycle (12‑15). It is also expressed in carcinomas of the kidney, ovary, and liver (8, 10, 11, 14).
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
  3. Inoue, T. et al. (1997) Dev. Biol. 183:183.
  4. Shimoyama, Y. et al. (2000) Biochem. J. 349:159.
  5. Shimoyama, Y. et al. (1999) J. Biol. Chem. 274:11987.
  6. Inoue, T. et al. (1998) Dev. Dyn. 211:338.
  7. Xiang Y.-Y. et al. (1994) Cancer Res. 54:3034.
  8. Shimoyama, Y. et al. (1995) Cancer Res. 55:2206.
  9. Cho, E.A. et al. (1998) Development 125:803.
  10. Paul, R. et al. (1997) Cancer Res. 57:2741.
  11. Shimazui, T. et al. (2000) Eur. Urol. 38:331.
  12. Ruan, G. et al. (2006) Mech. Dev. 123:881.
  13. Mbalaviele, G.  et al. (1998) J. Cell Biol.  141:1467.
  14. Sellar, G.C. et al. (2001) Cancer Res.  61:6977.
  15. Getsios, S. et al. (1998) Dev. Dyn. 211:238.

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